TBS: Difference between revisions
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(New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl {|border="1" |+ '''...) |
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Revision as of 05:18, 1 April 2008
Purpose
- TBS stands for tris-buffered saline and is used in many protocols for washing
Recipe
- Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
| for 1 liter with stock solutions |
|---|
| 100 ml 1M Tris-HCl pH 7.5 |
| 150 ml 5M NaCl |
| 750 ml ddH2O |
- Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again
Contact
- Who has experience using this material?
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