TBS

Purpose

• TBS stands for tris-buffered saline and is used in many protocols for washing

Recipe

• Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl

5xTBS

for 1 liter with stock solutions
100 ml 1M Tris-HCl pH 7.5
150 ml 5M NaCl
750 ml ddH2O

• Prepare and sterilize filter. Store at 4°C. After diluting the 5x to 1x filter again

• TBST: For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
  • Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
  • Usual concentration of Triton X-100 in TBST is 0.1%.