TBS - Revision history

Etienne Robillard: /* Recipe */

2012-12-11T00:19:21Z

Recipe

←Older revision		Revision as of 00:19, 11 December 2012
Line 18:		Line 18:
	\|}		\|}

-	*Prepare and ~~sterile~~ filter. Store at 4°C. After diluting the 5x to 1x filter again	+	*Prepare and sterilize filter. Store at 4°C. After diluting the 5x to 1x filter again

	*'''[[TBST]]''': For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.		*'''[[TBST]]''': For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.

Torsten Waldminghaus at 14:42, 12 January 2009

2009-01-12T14:42:31Z

←Older revision		Revision as of 14:42, 12 January 2009
Line 20:		Line 20:
	*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again		*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again

-	*'''[[TBST]]''': For some applications one adds Tween 20 or ~~TritonX100~~ to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.	+	*'''[[TBST]]''': For some applications one adds Tween 20 or Triton X-100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.		**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
-	**Usual concentration of ~~TritonX100~~ in TBST is 0.1%.	+	**Usual concentration of Triton X-100 in TBST is 0.1%.

Torsten Waldminghaus at 15:53, 2 April 2008

2008-04-02T15:53:24Z

←Older revision		Revision as of 15:53, 2 April 2008
Line 20:		Line 20:
	*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again		*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again

-	*'''TBST''': For some applications one adds Tween 20 to TBS which makes TBST.	+	*'''[[TBST]]''': For some applications one adds Tween 20 or TritonX100 to TBS which makes TBST (people use the name TBST for both, probably it's lab dependent.
	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.		**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
		+	**Usual concentration of TritonX100 in TBST is 0.1%.

Torsten Waldminghaus at 15:43, 2 April 2008

2008-04-02T15:43:08Z

←Older revision		Revision as of 15:43, 2 April 2008
Line 19:		Line 19:

	*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again		*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again
		+
		+	*'''TBST''': For some applications one adds Tween 20 to TBS which makes TBST.
		+	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
		+


-	~~==Contact==~~
-	*Who has experience using this material?


-	~~Add any other categories that may be relevant to your page. We use categories like tags.~~
	[[Category:Material]] [[Category:Buffers]]		[[Category:Material]] [[Category:Buffers]]

Torsten Waldminghaus: New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl {|border="1" |+ '''...

2008-04-01T12:18:44Z

New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl {|border="1" |+ '''...

New page

==Purpose==
* TBS stands for tris-buffered saline and is used in many protocols for washing

==Recipe==

*Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl

{|border="1"
|+ '''5xTBS'''
! for 1 liter with stock solutions
|-
|100 ml 1M Tris-HCl pH 7.5
|-
|150 ml 5M NaCl
|-
|750 ml ddH<sub>2</sub>O
|}

*Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again

==Contact==
*Who has experience using this material?

Add any other categories that may be relevant to your page. We use categories like tags.
[[Category:Material]] [[Category:Buffers]]