TBS

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Purpose

  • TBS stands for tris-buffered saline and is used in many protocols for washing


Recipe

  • Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
5xTBS
for 1 liter with stock solutions
100 ml 1M Tris-HCl pH 7.5
150 ml 5M NaCl
750 ml ddH2O
  • Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again


Contact

  • Who has experience using this material?


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