TFB II: Difference between revisions

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(New page: DH5α Competent Cells {|border="1" |+TFB II !Reagent !!F.W. !!100 ml |- !10 mM MOPS |209.3 ||0.209 |- !75 mM CaCl<sub>2</sub> |147 ||1.102 |- !10 mM RbCl |120.9 ||0.121 |- !15% Gly...)
 
(No difference)

Latest revision as of 13:57, 3 September 2009

DH5α Competent Cells

TFB II
Reagent F.W. 100 ml
10 mM MOPS 209.3 0.209
75 mM CaCl2 147 1.102
10 mM RbCl 120.9 0.121
15% Glycerol N/A 15 ml

pH 6.5 w/ KOH. Filter sterilize.