TFB II

From OpenWetWare
Revision as of 13:57, 3 September 2009 by Matthew Shepherd (talk | contribs) (New page: DH5α Competent Cells {|border="1" |+TFB II !Reagent !!F.W. !!100 ml |- !10 mM MOPS |209.3 ||0.209 |- !75 mM CaCl<sub>2</sub> |147 ||1.102 |- !10 mM RbCl |120.9 ||0.121 |- !15% Gly...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

DH5α Competent Cells

TFB II
Reagent F.W. 100 ml
10 mM MOPS 209.3 0.209
75 mM CaCl2 147 1.102
10 mM RbCl 120.9 0.121
15% Glycerol N/A 15 ml

pH 6.5 w/ KOH. Filter sterilize.