TSS

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To make 50 mL:
To make 50 mL:
*5g PEG 8000
*5g PEG 8000
-
*1.5 mL 1M MgCl2
+
*1.5 mL 1M MgCl<sub>2</sub> (or 0.30g MgCl<sub>2</sub>*6H<sub>2</sub>0)
*2.5 mL DMSO
*2.5 mL DMSO
*LB to 50 mL
*LB to 50 mL
Store at 4C
Store at 4C

Revision as of 11:35, 7 June 2005

To make 50 mL:

  • 5g PEG 8000
  • 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
  • 2.5 mL DMSO
  • LB to 50 mL

Store at 4C

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