TSS

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==Uses==
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TSS buffer is used to make [[Preparing chemically competent cells|chemically competent cells]].
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==Preparation==
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To make 50 mL:
To make 50 mL:
*5g PEG 8000
*5g PEG 8000
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*Add LB to 50 mL
*Add LB to 50 mL
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Filter sterilize (0.22 μm filter) and store at 4˚C or -20˚C.
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Filter sterilize (0.22 μm filter)
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==Storage==
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Store at 4˚C or -20˚C.

Revision as of 13:14, 16 January 2006

Uses

TSS buffer is used to make chemically competent cells.

Preparation

To make 50 mL:

  • 5g PEG 8000
  • 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
  • 2.5 mL DMSO
  • Add LB to 50 mL

Filter sterilize (0.22 μm filter)

Storage

Store at 4˚C or -20˚C.

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