TSS
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(Difference between revisions)
| Line 1: | Line 1: | ||
| + | ==Uses== | ||
| + | TSS buffer is used to make [[Preparing chemically competent cells|chemically competent cells]]. | ||
| + | |||
| + | ==Preparation== | ||
| + | |||
To make 50 mL: | To make 50 mL: | ||
*5g PEG 8000 | *5g PEG 8000 | ||
| Line 5: | Line 10: | ||
*Add LB to 50 mL | *Add LB to 50 mL | ||
| - | Filter sterilize (0.22 μm filter) | + | Filter sterilize (0.22 μm filter) |
| + | |||
| + | ==Storage== | ||
| + | Store at 4˚C or -20˚C. | ||
Revision as of 13:14, 16 January 2006
Uses
TSS buffer is used to make chemically competent cells.
Preparation
To make 50 mL:
- 5g PEG 8000
- 1.5 mL 1M MgCl2 (or 0.30g MgCl2*6H20)
- 2.5 mL DMSO
- Add LB to 50 mL
Filter sterilize (0.22 μm filter)
Storage
Store at 4˚C or -20˚C.
