T broth: Difference between revisions
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Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen. | Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen. | ||
==Source== | ==Source== | ||
Studier, FW Virology '''39''':562 (1969) Link: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4902069&query_hl=1 Pubmed] | |||
Revision as of 10:40, 12 September 2005
Summary
T-Broth (not to be confused with Terrific broth)
Ingredients
- 10 g Bacto-tryptone
- 5 g NaCl
Protocol
- Mix dry ingredients and add distilled water up to 1 Liter
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- 250°F, 22psi, 30 minutes
Notes: We do not pH medium when we make it on the fly. However, if it is really important, pH the medium to 7.0. We usually obtain this from the kitchen.
Source
Studier, FW Virology 39:562 (1969) Link: Pubmed
