Table of c5.0 Working Stocks

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(Working Stock)
(Working Stock)
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| c5.0C||latch design 2, inside aptamers||x||-||-||x||-||-||x||-||-||-||-||x||x||x||x||x||x||-||-||-
| c5.0C||latch design 2, inside aptamers||x||-||-||x||-||-||x||-||-||-||-||x||x||x||x||x||x||-||-||-
|-
|-
-
| c5.0D||latch design 2, outside aptamers||x||x||x||-||x||x||-||-||-||-||-||x||x||x||x||x||x||-||-||-
+
| c5.0D||latch design 2, outside aptamers||x||-||-||-||x||x||-||-||-||-||-||x||x||x||x||x||x||-||-||-
|-
|-
| c5.0E(b)||no latches, outside biotin||172uL||-||-||-||-||5||-||3||3||5||-||-||-||-||-||-||-||-||-||-
| c5.0E(b)||no latches, outside biotin||172uL||-||-||-||-||5||-||3||3||5||-||-||-||-||-||-||-||-||-||-

Revision as of 11:48, 14 November 2006

Working Stock

NB: Noticed that c5.0A row included pre-working stocks 8 and 9, erroneously. These are biotinylated versions of 6 and 7, and should not have been included in previous stocks. Also, pre-working stocks 20 and 21 were erroneously included - these are displacement oligos, to be (putatively) added after latches are folded, with the lids present. Also, 10+11 is redundant with 6 and 7 - if latches are necessary, add 10+11; if no latches are desired, use 6 and 7. ---TC, 8.23.06

Stock IDExperiment12344L55L678910+111213+141516+1718192021
c5.0Ano latches, no aptamersxxxx-x-x---xxxxxxx--
c5.0A (lidless)no latches, no aptamers172uL--5-5-33-----------
c5.0Blatch design 2, no aptamersx--x-x-----xxxxxx---
c5.0Clatch design 2, inside aptamersx--x--x----xxxxxx---
c5.0Dlatch design 2, outside aptamersx---xx-----xxxxxx---
c5.0E(b)no latches, outside biotin172uL----5-335----------
c5.0F(b)no latches, intside biotin172uL--5---33-5---------
c5.0E(b)rno latches, outside biotin172uL----5-338----------
c5.0F(b)rno latches, intside biotin172uL--5---33-8---------

Pre-Working Stocks

Description Plate Locations Total
c5.0.1: core barrel oligosbox_v5_A; box_v5_B rows A-F (1-12), row G (1-4)172
c5.0.2: top lid oligosbox_v5_C; box_v5_D row A (1-2)98
c5.0.3: bottom lid oligosbox_v5_D row A (3-12), rows B-H (1-12); box_v5_E row A (1-4)98
c5.0.4: barrel oligos @ outside aptamers -aptamersbox_v5_E row A (5-9)5
c5.0.4L: barrel oligos @ outside aptamers+ligandbox_v5_F row F (1-10)10
c5.0.5: barrel oligos @ inside aptamers -aptamersbox_v5_E row A (10-12), row B (1-2)5
c5.0.5L: barrel oligos @ inside aptamers+ligandbox_v5_F row F (11-12), row G (1-10)12
c5.0.6: barrel oligos @ top latches -latchesbox_v5_E row B (3-5)3
c5.0.7: barrel oligos @ bottom latches -latchesbox_v5_E row B (6-8)3
c5.0.8: barrel oligos @ outside aptamers +biotintubes c5.0.8.1-55
c5.0.9: barrel oligos @ inside aptamers +biotintubes c5.0.9.1-55
c5.0.8r: revised barrel oligos @ outside aptamers +biotintubes c5.0.8.1ra & b, c5.0.8.2ra & b, c5.0.8.5ra &b, plus c5.0.8.3 and c5.0.8.48
c5.0.9r: revised barrel oligos @ inside aptamers +biotintubes c5.0.9.1ra & b - c5.0.9.5ra & b8
c5.0.10+c5.0.11: barrel oligos @ latches (for latches)box_v5_F row A (1-9)9
c5.0.12: barrel latch zippersbox_v5_F row A (10-12), row B (1-3)6
c5.0.13+c5.0.14: top lid latchesbox_v5_F row B (4-8)5
c5.0.15: top lid latch zippersbox_v5_F row B (9-11)3
c5.0.16+c5.0.17:bottom lid latchesbox_v5_F row B (12), row C (1-5)6
c5.0.18: bottom lid latch zippersbox_v5_F row C (6-8)3
c5.0.19: barrel displacement oligosbox_v5_F row C (9-12), row D (1-2)6
c5.0.20: top lid displacement oligosbox_v5_F row D (3-5)3
c5.0.21: bottom lid displacement oligosbox_v5_F row D (6-8)3

Totals

Working Stocks Description Pre-Working Stocks Water Total
c5.0Ano latches, no aptamers, no lids1 (86 μL), 4 (2.5 μL), 5 (2.5 μL), 6 (1.5 μL), 7 (1.5 μL)106 μL200 μL
c5.0A.lidsno latches, no aptamers2 (49 μL), 3 (49 μL)102 μL200 μL
c5.0C.corelatch 2, in aptamers1 (86 μL), 2 (49 μL), 3 (49 μL), 4 (2.5 μL), 5L (6 μL)--
c5.0C.latcheslatch 2, in aptamers10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL))--
c5.0D.corelatch 2, out aptamers1 (86 μL), 2 (49 μL), 3 (49 μL), 4L (5 μL), 5 (2.5 μL)--
c5.0D.latcheslatch 2, out aptamers10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL))--
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