Working Stock
NB: Noticed that c5.0A row included pre-working stocks 8 and 9, erroneously. These are biotinylated versions of 6 and 7, and should not have been included in previous stocks. Also, pre-working stocks 20 and 21 were erroneously included - these are displacement oligos, to be (putatively) added after latches are folded, with the lids present. Nor does it make any true sense to fold c.5.0A with any lid accoutrements (ie. pre-working stocks 12, 13+14, 15, 16+17, 18, 19) without the lid scaffold/folded lid being in the solution. Also, 10+11 is redundant with 6 and 7 - if latches are necessary, add 10+11; if no latches are desired, use 6 and 7. ---TC, 8.23.06
Stock ID |
Experiment |
1 |
2 |
3 |
4 |
4L |
5 |
5L |
6 |
7 |
8 |
9 |
10+11 |
12 |
13+14 |
15 |
16+17 |
18 |
19 |
20 |
21
|
c5.0A |
no latches, no aptamers |
x |
x |
x |
x |
- |
x |
- |
x |
- |
- |
x |
x |
x |
x |
x |
x |
x |
x |
- |
-
|
c5.0A (lidless) |
no latches, no aptamers |
172uL |
- |
- |
5 |
- |
5 |
- |
3 |
3 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
- |
-
|
c5.0B |
latch design 2, no aptamers |
x |
x |
x |
x |
- |
x |
- |
- |
- |
- |
- |
x |
x |
x |
x |
x |
x |
- |
- |
-
|
c5.0C |
latch design 2, inside aptamers |
x |
x |
x |
x |
- |
- |
x |
- |
- |
- |
- |
x |
x |
x |
x |
x |
x |
- |
- |
-
|
c5.0D |
latch design 2, outside aptamers |
x |
x |
x |
- |
x |
x |
- |
- |
- |
- |
- |
x |
x |
x |
x |
x |
x |
- |
- |
-
|
c5.0E(b) |
no latches, outside biotin |
172uL |
- |
- |
- |
- |
5 |
- |
3 |
3 |
5 |
- |
- |
- |
- |
- |
- |
- |
- |
- |
-
|
c5.0F(b) |
no latches, intside biotin |
172uL |
- |
- |
5 |
- |
- |
- |
3 |
3 |
- |
5 |
- |
- |
- |
- |
- |
- |
- |
- |
-
|
Pre-Working Stocks
Description
|
Plate Locations
|
Total
|
c5.0.1: core barrel oligos |
box_v5_A; box_v5_B rows A-F (1-12), row G (1-4) |
172
|
c5.0.2: top lid oligos |
box_v5_C; box_v5_D row A (1-2) |
98
|
c5.0.3: bottom lid oligos |
box_v5_D row A (3-12), rows B-H (1-12); box_v5_E row A (1-4) |
98
|
c5.0.4: barrel oligos @ outside aptamers -aptamers |
box_v5_E row A (5-9) |
5
|
c5.0.4L: barrel oligos @ outside aptamers+ligand |
box_v5_F row F (1-10) |
10
|
c5.0.5: barrel oligos @ inside aptamers -aptamers |
box_v5_E row A (10-12), row B (1-2) |
5
|
c5.0.5L: barrel oligos @ inside aptamers+ligand |
box_v5_F row F (11-12), row G (1-10) |
12
|
c5.0.6: barrel oligos @ top latches -latches |
box_v5_E row B (3-5) |
3
|
c5.0.7: barrel oligos @ bottom latches -latches |
box_v5_E row B (6-8) |
3
|
c5.0.8: barrel oligos @ outside aptamers +biotin |
tubes c5.0.8.1-5 |
5
|
c5.0.9: barrel oligos @ inside aptamers +biotin |
tubes c5.0.9.1-5 |
5
|
c5.0.10+c5.0.11: barrel oligos @ latches (for latches) |
box_v5_F row A (1-9) |
9
|
c5.0.12: barrel latch zippers |
box_v5_F row A (10-12), row B (1-3) |
6
|
c5.0.13+c5.0.14: top lid latches |
box_v5_F row B (4-8) |
5
|
c5.0.15: top lid latch zippers |
box_v5_F row B (9-11) |
3
|
c5.0.16+c5.0.17:bottom lid latches |
box_v5_F row B (12), row C (1-5) |
6
|
c5.0.18: bottom lid latch zippers |
box_v5_F row C (6-8) |
3
|
c5.0.19: barrel displacement oligos |
box_v5_F row C (9-12), row D (1-2) |
6
|
c5.0.20: top lid displacement oligos |
box_v5_F row D (3-5) |
3
|
c5.0.21: bottom lid displacement oligos |
box_v5_F row D (6-8) |
3
|
Totals
Working Stocks
|
Description
|
Pre-Working Stocks
|
Water
|
Total
|
c5.0A |
no latches, no aptamers, no lids |
1 (86 μL), 4 (2.5 μL), 5 (2.5 μL), 6 (1.5 μL), 7 (1.5 μL) |
106 μL |
200 μL
|
c5.0A.lids |
no latches, no aptamers |
2 (49 μL), 3 (49 μL) |
102 μL |
200 μL
|
c5.0C.core |
latch 2, in aptamers |
1 (86 μL), 2 (49 μL), 3 (49 μL), 4 (2.5 μL), 5L (6 μL) |
- |
-
|
c5.0C.latches |
latch 2, in aptamers |
10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL)) |
- |
-
|
c5.0D.core |
latch 2, out aptamers |
1 (86 μL), 2 (49 μL), 3 (49 μL), 4L (5 μL), 5 (2.5 μL) |
- |
-
|
c5.0D.latches |
latch 2, out aptamers |
10+11 (4.5 μL), 12 (3 μL), 13+14 (2.5 μL), 15 (1.5 μL), 16+17 (3 μL), 18 (1.5 μL)) |
- |
-
|