Talk:20.109(F07):Start-up genome engineering: Difference between revisions

Revision as of 19:29, 11 September 2007

oligo name	oligo number	oligo sequence	primer record	enzymes added with insert
TeacherOligo_top	NO196	5' GAT CTG CGC ACG CGT	teacher sample oligo record	FspI and MluI
TeacherOligo_btm	NO197	5'G,ATC,ACG,CGT,GCG,CA
M13KO7seq_2101_fwd	NO198	5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3'
M13KO7seq_2151_fwd	NO199	5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3'

oligo name	oligo number	oligo sequence	primer record	enzymes added with insert
TRpink_top	NO	5’ GAT CTC GAC GGC GCC 3’	Oligo design record	Sal I
TRpink_btm	NO	5’ G ATC GGC GCC GTC GA 3’	Oligo design record	Sfo I
TRyellow_top	NO	5' GAT CTA GAT ATC 3'	Yellow Insert Design	Xba I, Eco RV
TRyellow_btm	NO	5' G ATC GAT ATC TA 3'	Yellow Insert Design
TRblue_top	NO	5' GAT CAG ATC TTA GGC GCC CGA 3'	Blue Insert Design	BglII
TRblue_btm	NO	5' G ATC TGC GGC GCC TAA GAT CT 3'	Blue Insert Design	SfoI
TRpurple_top	NO	5' GAT CTG ATC AGT ACT 3'
TRpurple_btm	NO	5' GAT CAG TAC TGA TCA 3'
TRred_top	NO	5' GAT CTT AAG GAC GTC	Red Insert Design	AflII ZraI
TRred_btm	NO	5' G ATC GAC GTC CTT AA
TRgreen_top	NO	GAT CGG TAC CAC GTC	Green Insert Design	KpnI BmgI
TRgreen_btm	NO	CC ATG GTG CAG CTA G

@@ Line 83: / Line 83: @@
 | TRpurple_top
 | NO
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+| 5' GAT CTG ATC AGT ACT 3'
 |
 |
@@ Line 89: / Line 89: @@
 | TRpurple_btm
 | NO
-|
+| 5' GAT CAG TAC TGA TCA 3'
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 |