Talk:20.109(F07):Start-up genome engineering: Difference between revisions
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| WFpurple_top | | WFpurple_top | ||
| NO | | NO | ||
| | | gat/cGA/ATT/CCA/CGT/C | ||
| | || [[Media:Day 1, Part 4.doc|Purple Insert Design]] | ||
| | |EcoRI, BmgBI | ||
|- | |- | ||
| WFpurple_btm | | WFpurple_btm | ||
| NO | | NO | ||
| | | ga/tcG/ACG/TGG/AAT/TC | ||
| | | | ||
| | | |
Revision as of 13:42, 12 September 2007
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
---|---|---|---|---|---|
TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
TRpink_top | NO | 5’ GAT CTC GAC GGC GCC 3’ | Oligo design record | Sal I |
TRpink_btm | NO | 5’ G ATC GGC GCC GTC GA 3’ | Oligo design record | Sfo I |
TRyellow_top | NO | 5' GAT CTA GAT ATC 3' edit:5'gat cta gaC GAt atc |
Yellow Insert Design | Xba I, Eco RV |
TRyellow_btm | NO | 5' G ATC GAT ATC TA 3' edit: 5'G atc gat atc gtc ta |
Yellow Insert edits! | |
TRblue_top | NO | 5' GAT CAG ATC TTA GGC GCC CGA 3' | Blue Insert Design | BglII |
TRblue_btm | NO | 5' G ATC TGC GGC GCC TAA GAT CT 3' | Blue Insert Design | SfoI |
TRpurple_top | NO | 5' GAT CTG ATC AGT ACT 3' | Purple Insert Design | BclI, ScaI |
TRpurple_btm | NO | 5' GAT CAG TAC TGA TCA 3' | ||
TRred_top | NO | 5' GAT CTT AAG GAC GTC | Red Insert Design | AflII ZraI |
TRred_btm | NO | 5' G ATC GAC GTC CTT AA | ||
TRgreen_top | NO | 5' GAT CGG TAC CAC GTC 3' | Green Insert Design | KpnI BmgI |
TRgreen_btm | NO | 5' G ATC GAC GTG GTA CC 3' |
W/F lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
WFpink_top | NO | |||
WFpink_btm | NO | |||
WFyellow_top | NO | |||
WFyellow_btm | NO | |||
WFblue_top | NO | |||
WFblue_btm | NO | |||
WFpurple_top | NO | gat/cGA/ATT/CCA/CGT/C | Purple Insert Design | EcoRI, BmgBI |
WFpurple_btm | NO | ga/tcG/ACG/TGG/AAT/TC | ||
WFred_top | NO | gat/cGA/ATT/CTT/GTT/AAC | Red Insert Design | EcoRI, HpaI |
WFred_btm | NO | g/atc/GTT/AAC/AAG/AAT/TC | ||
WFgreen_top | NO | |||
WFgreen_btm | NO |
[[Media:Media:Example.oggMedia:Example.ogg]]