Talk:20.109(F07): Luciferase assays and RNA prep: Difference between revisions
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Jessie Wang (talk | contribs) |
Justin Tan (talk | contribs) |
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| Line 18: | Line 18: | ||
| No DNA | | No DNA | ||
| 0.0, .083 | | 0.0, .083 | ||
| .053, .054 | | ff: 0.008, 0.009, r: 0.053, 0.054 | ||
| 394.3, 470.9 | | 394.3, 470.9 | ||
| 11.47, 9.00 | | 11.47, 9.00 | ||
| Line 26: | Line 26: | ||
| plasmid only | | plasmid only | ||
| 11.12, 406.2 | | 11.12, 406.2 | ||
| 3826, 3299 | | ff: 119.0, 109.3, R: 3826, 3299 | ||
| 5864, 9516 | | 5864, 9516 | ||
| 29.50, 30.04 | | 29.50, 30.04 | ||
| Line 34: | Line 34: | ||
| siRNA only | | siRNA only | ||
| 0.009, .049 | | 0.009, .049 | ||
| .063, .051 | | ff: 0.012, 0.013, R: .063, .051 | ||
| 354, 467.6 | | 354, 467.6 | ||
| 4.40, 10.40 | | 4.40, 10.40 | ||
| Line 42: | Line 42: | ||
| plasmid+siRNA (validated) | | plasmid+siRNA (validated) | ||
| 23.21, 843.7 | | 23.21, 843.7 | ||
| 136.4, 177.7 | | ff: 61.80, 86.20, R: 136.4, 177.7 | ||
| 0.058, 0.074 | | 0.058, 0.074 | ||
| 3.86, 4.05 | | 3.86, 4.05 | ||
| Line 50: | Line 50: | ||
| plasmid+siRNA (scrambled) | | plasmid+siRNA (scrambled) | ||
| .521, 19.31 | | .521, 19.31 | ||
| | | ff: 76.87, 88.13, R: 2184, 2819 | ||
| 8705, 4133 | | 8705, 4133 | ||
| 32.29, 31.25 | | 32.29, 31.25 | ||
| Line 58: | Line 58: | ||
| plasmid+siRNA (experimental) | | plasmid+siRNA (experimental) | ||
| 11.14, 354.3 | | 11.14, 354.3 | ||
| 1170, 1209 | | ff: 124.2, 124.2, R: 1170, 1209 | ||
| 0.113, 0.114 | | 0.113, 0.114 | ||
| 15.58, 17.75 | | 15.58, 17.75 | ||
Revision as of 13:09, 23 October 2007
Luciferase Assay Data
Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.
T/R lab
| Sample | Green | Purple | Red | Blue | Pink | Yellow |
|---|---|---|---|---|---|---|
| No DNA | 0.0, .083 | ff: 0.008, 0.009, r: 0.053, 0.054 | 394.3, 470.9 | 11.47, 9.00 | 0.012, 0.0515 | ff: 0.0103, 0.0031; R: 0.0903, 0.0616 |
| plasmid only | 11.12, 406.2 | ff: 119.0, 109.3, R: 3826, 3299 | 5864, 9516 | 29.50, 30.04 | 23.49, 682.95 | ff: 181.9, 195.1; R: 5175, 5496 |
| siRNA only | 0.009, .049 | ff: 0.012, 0.013, R: .063, .051 | 354, 467.6 | 4.40, 10.40 | 0.006, 0.0615 | ff: 0.0068, 0.0257; R: 0.0866, 0.0721 |
| plasmid+siRNA (validated) | 23.21, 843.7 | ff: 61.80, 86.20, R: 136.4, 177.7 | 0.058, 0.074 | 3.86, 4.05 | 19.56, 61.78 | ff: 245.1, 255.8; R: 443.5, 438.5 |
| plasmid+siRNA (scrambled) | .521, 19.31 | ff: 76.87, 88.13, R: 2184, 2819 | 8705, 4133 | 32.29, 31.25 | 20.67, 603.95 | ff: 229.3, 220.0; R: 6976, 7308 |
| plasmid+siRNA (experimental) | 11.14, 354.3 | ff: 124.2, 124.2, R: 1170, 1209 | 0.113, 0.114 | 15.58, 17.75 | 17.3, 182.4 | ff: 223.1, 233.2; R: 748.7, 717.1 |
W/F lab
| Sample | Green | Purple | Red | Blue | Pink | Yellow |
|---|---|---|---|---|---|---|
| No DNA | --. 0.042 | .048, .050 | ff 0.000,0.288 R 0.486,0.183 | ff 0.006,0.011 R 0.019,0.057 | ||
| plasmid only | 479.6, 206.2 | 2132, 17.75 | ff 53.18,60.96 R 138.1,66.05 | ff 2.276,40.13 R 10.41,131.7 | ||
| siRNA only | 0.055, 0.058 | .056, .013 | ff 0.047,0.117 R 0.241,0.299 | ff 0.011,0.012 R 0.024,0.140 | ||
| plasmid+siRNA (validated) | 210.6, 130.5 | 158.3, 0.963 | ff 57.02,57.56 R 28.75,13.65 | ff 5.476,5.378 R 12.73,28.36 | ||
| plasmid+siRNA (scrambled) | 1749, 1478 | 1167, 3.408 | ff 58.33,59.67 R 149.8,42.17 | ff 6.211,55.10 R 48.15,2427 | ||
| plasmid+siRNA (experimental) | 113, 107.1 | 202.9, 0.779 | ff 69.26,63.04 R 45.68,57.43 | ff 4.872,47.39 R 3.998,210.4 |
