Talk:20.109(F07): Luciferase assays and RNA prep: Difference between revisions
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Line 17: | Line 17: | ||
|- | |- | ||
| No DNA | | No DNA | ||
| | | 0.0, .083 | ||
| .053, .054 | | ff: 0.008, 0.009, R: 0.053, 0.054 | ||
| | | 394.3, 470.9 | ||
| | | ff: 0.017,0.005, R: 0.195, 0.045 | ||
| 0.012, 0. | | ff: 0.012, 0.012; R: 0.049, 0.054 | ||
| | | ff: 0.0103, 0.0031; R: 0.0903, 0.0616 | ||
|- | |- | ||
| plasmid only | | plasmid only | ||
| | | 11.12, 406.2 | ||
| 3826, 3299 | | ff: 119.0, 109.3, R: 3826, 3299 | ||
| | | 5864, 9516 | ||
| | | ff: 74.52, 66.88, R: 2198, 2009 | ||
| | | ff: 21.42, 25.56; R: 618.0, 747.9 | ||
| | | ff: 181.9, 195.1; R: 5175, 5496 | ||
|- | |- | ||
| siRNA only | | siRNA only | ||
| | | 0.009, .049 | ||
| .063, .051 | | ff: 0.012, 0.013, R: .063, .051 | ||
| | | 354, 467.6 | ||
| | | ff: 0.010, 0.010 R: 0.044, 0.104 | ||
| 0. | | ff: 0.007, 0.005; R: 0.064, 0.059 | ||
| | | ff: 0.0068, 0.0257; R: 0.0866, 0.0721 | ||
|- | |- | ||
| plasmid+siRNA (validated) | | plasmid+siRNA (validated) | ||
| | | 23.21, 843.7 | ||
| 136.4, 177.7 | | ff: 61.80, 86.20, R: 136.4, 177.7 | ||
| | | 0.058, 0.074 | ||
| | | ff: 48.06 78.31, R: 185.7, 317. 2 | ||
| | | ff: 29.88, 14.24; R: 81.25, 42.32 | ||
| | | ff: 245.1, 255.8; R: 443.5, 438.5 | ||
|- | |- | ||
| plasmid+siRNA (scrambled) | | plasmid+siRNA (scrambled) | ||
| | | .521, 19.31 | ||
| | | ff: 76.87, 88.13, R: 2184, 2819 | ||
| | | 8705, 4133 | ||
| | | ff: 86.87, 66.71 R: 2805, 2085 | ||
| | | ff: 26.32, 15.02; R: 760.3, 447.6 | ||
| | | ff: 229.3, 220.0; R: 6976, 7308 | ||
|- | |- | ||
| plasmid+siRNA (experimental) | | plasmid+siRNA (experimental) | ||
| | | 11.14, 354.3 | ||
| 1170, 1209 | | ff: 124.2, 124.2, R: 1170, 1209 | ||
| | | 0.113, 0.114 | ||
| | | ff: 120.2, 74.77, R: 1881, 1327 | ||
| | | ff: 22.33, 12.16; R: 237.1, 127.7 | ||
| ff: 223.1, 233.2; R: 748.7, 717.1 | |||
| | |||
|} | |} | ||
</center> | </center> | ||
Line 69: | Line 68: | ||
===W/F lab=== | ===W/F lab=== | ||
<center> | <center> | ||
{| border="1" | {| border="1" | ||
! Sample | ! Sample | ||
! Green | ! Green | ||
Line 81: | Line 80: | ||
| --. 0.042 | | --. 0.042 | ||
| .048, .050 | | .048, .050 | ||
| | | ff 0.059, 0.096; R 0.658, 1.164 | ||
| | | ff 0.000,0.288 R 0.486,0.183 | ||
| | | ff 0.006,0.011 R 0.019,0.057 | ||
| | | | ||
|- | |- | ||
Line 89: | Line 88: | ||
| 479.6, 206.2 | | 479.6, 206.2 | ||
| 2132, 17.75 | | 2132, 17.75 | ||
| | |(1/10 dil) ff 9.332, 78.35; R 252.1, 1773 | ||
| | | ff 53.18,60.96 R 138.1,66.05 | ||
| | | ff 2.276,40.13 R 10.41,1317 | ||
| | | | ||
|- | |- | ||
Line 97: | Line 96: | ||
| 0.055, 0.058 | | 0.055, 0.058 | ||
|.056, .013 | |.056, .013 | ||
| | |ff 0.080, 0.120; R 0.481, 0.575 | ||
| | | ff 0.047,0.117 R 0.241,0.299 | ||
| | | ff 0.011,0.012 R 0.024,0.140 | ||
| | | | ||
|- | |- | ||
Line 105: | Line 104: | ||
| 210.6, 130.5 | | 210.6, 130.5 | ||
| 158.3, 0.963 | | 158.3, 0.963 | ||
| | | ff 781.6, 731.0; R 2114, 2537 | ||
| | | ff 57.02,57.56 R 28.75,13.65 | ||
| | | ff 5.476,5.378 R 12.73,28.36 | ||
| | | | ||
|- | |- | ||
Line 113: | Line 112: | ||
| 1749, 1478 | | 1749, 1478 | ||
| 1167, 3.408 | | 1167, 3.408 | ||
| | | (1/10 dil) ff 162.2, 85.75; R 5424, 2225 | ||
| | | ff 58.33,59.67 R 149.8,42.17 | ||
| | | ff 6.211,55.10 R 48.15,2427 | ||
| | | | ||
|- | |- | ||
Line 121: | Line 120: | ||
| 113, 107.1 | | 113, 107.1 | ||
| 202.9, 0.779 | | 202.9, 0.779 | ||
| | | ff 926.4, 644.1; R 2925, 1880 | ||
| | | ff 69.26,63.04 R 45.68,57.43 | ||
| | | ff 4.872,47.39 R 3.998,210.4 | ||
| | | | ||
|} | |} | ||
</center> | </center> |
Latest revision as of 19:14, 1 November 2007
Luciferase Assay Data
Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.
T/R lab
Sample | Green | Purple | Red | Blue | Pink | Yellow |
---|---|---|---|---|---|---|
No DNA | 0.0, .083 | ff: 0.008, 0.009, R: 0.053, 0.054 | 394.3, 470.9 | ff: 0.017,0.005, R: 0.195, 0.045 | ff: 0.012, 0.012; R: 0.049, 0.054 | ff: 0.0103, 0.0031; R: 0.0903, 0.0616 |
plasmid only | 11.12, 406.2 | ff: 119.0, 109.3, R: 3826, 3299 | 5864, 9516 | ff: 74.52, 66.88, R: 2198, 2009 | ff: 21.42, 25.56; R: 618.0, 747.9 | ff: 181.9, 195.1; R: 5175, 5496 |
siRNA only | 0.009, .049 | ff: 0.012, 0.013, R: .063, .051 | 354, 467.6 | ff: 0.010, 0.010 R: 0.044, 0.104 | ff: 0.007, 0.005; R: 0.064, 0.059 | ff: 0.0068, 0.0257; R: 0.0866, 0.0721 |
plasmid+siRNA (validated) | 23.21, 843.7 | ff: 61.80, 86.20, R: 136.4, 177.7 | 0.058, 0.074 | ff: 48.06 78.31, R: 185.7, 317. 2 | ff: 29.88, 14.24; R: 81.25, 42.32 | ff: 245.1, 255.8; R: 443.5, 438.5 |
plasmid+siRNA (scrambled) | .521, 19.31 | ff: 76.87, 88.13, R: 2184, 2819 | 8705, 4133 | ff: 86.87, 66.71 R: 2805, 2085 | ff: 26.32, 15.02; R: 760.3, 447.6 | ff: 229.3, 220.0; R: 6976, 7308 |
plasmid+siRNA (experimental) | 11.14, 354.3 | ff: 124.2, 124.2, R: 1170, 1209 | 0.113, 0.114 | ff: 120.2, 74.77, R: 1881, 1327 | ff: 22.33, 12.16; R: 237.1, 127.7 | ff: 223.1, 233.2; R: 748.7, 717.1 |
W/F lab
Sample | Green | Purple | Red | Blue | Pink | Yellow |
---|---|---|---|---|---|---|
No DNA | --. 0.042 | .048, .050 | ff 0.059, 0.096; R 0.658, 1.164 | ff 0.000,0.288 R 0.486,0.183 | ff 0.006,0.011 R 0.019,0.057 | |
plasmid only | 479.6, 206.2 | 2132, 17.75 | (1/10 dil) ff 9.332, 78.35; R 252.1, 1773 | ff 53.18,60.96 R 138.1,66.05 | ff 2.276,40.13 R 10.41,1317 | |
siRNA only | 0.055, 0.058 | .056, .013 | ff 0.080, 0.120; R 0.481, 0.575 | ff 0.047,0.117 R 0.241,0.299 | ff 0.011,0.012 R 0.024,0.140 | |
plasmid+siRNA (validated) | 210.6, 130.5 | 158.3, 0.963 | ff 781.6, 731.0; R 2114, 2537 | ff 57.02,57.56 R 28.75,13.65 | ff 5.476,5.378 R 12.73,28.36 | |
plasmid+siRNA (scrambled) | 1749, 1478 | 1167, 3.408 | (1/10 dil) ff 162.2, 85.75; R 5424, 2225 | ff 58.33,59.67 R 149.8,42.17 | ff 6.211,55.10 R 48.15,2427 | |
plasmid+siRNA (experimental) | 113, 107.1 | 202.9, 0.779 | ff 926.4, 644.1; R 2925, 1880 | ff 69.26,63.04 R 45.68,57.43 | ff 4.872,47.39 R 3.998,210.4 |