Talk:20.109(F07): Luciferase assays and RNA prep: Difference between revisions
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| Line 17: | Line 17: | ||
|- | |- | ||
| No DNA | | No DNA | ||
| | | 0.0, .083 | ||
| .053, .054 | | .053, .054 | ||
| 394.3, 470.9 | | 394.3, 470.9 | ||
| Line 25: | Line 25: | ||
|- | |- | ||
| plasmid only | | plasmid only | ||
| | | 11.12, 406.2 | ||
| 3826, 3299 | | 3826, 3299 | ||
| 5864, 9516 | | 5864, 9516 | ||
| Line 33: | Line 33: | ||
|- | |- | ||
| siRNA only | | siRNA only | ||
| | | 0.009, .049 | ||
| .063, .051 | | .063, .051 | ||
| 354, 467.6 | | 354, 467.6 | ||
| Line 41: | Line 41: | ||
|- | |- | ||
| plasmid+siRNA (validated) | | plasmid+siRNA (validated) | ||
| | | 23.21, 843.7 | ||
| 136.4, 177.7 | | 136.4, 177.7 | ||
| 0.058, 0.074 | | 0.058, 0.074 | ||
| Line 49: | Line 49: | ||
|- | |- | ||
| plasmid+siRNA (scrambled) | | plasmid+siRNA (scrambled) | ||
| | | .521, 19.31 | ||
| 2184, 2819 | | 2184, 2819 | ||
| 8705, 4133 | | 8705, 4133 | ||
| Line 57: | Line 57: | ||
|- | |- | ||
| plasmid+siRNA (experimental) | | plasmid+siRNA (experimental) | ||
| | | 11.14, 354.3 | ||
| 1170, 1209 | | 1170, 1209 | ||
| 0.113, 0.114 | | 0.113, 0.114 | ||
Revision as of 15:08, 22 October 2007
Luciferase Assay Data
Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.
T/R lab
| Sample | Green | Purple | Red | Blue | Pink | Yellow | |
|---|---|---|---|---|---|---|---|
| No DNA | 0.0, .083 | .053, .054 | 394.3, 470.9 | 11.47, 9.00 | 0.012, 0.0515 | ||
| plasmid only | 11.12, 406.2 | 3826, 3299 | 5864, 9516 | 29.50, 30.04 | 23.49, 682.95 | ||
| siRNA only | 0.009, .049 | .063, .051 | 354, 467.6 | 4.40, 10.40 | 0.006, 0.0615 | ||
| plasmid+siRNA (validated) | 23.21, 843.7 | 136.4, 177.7 | 0.058, 0.074 | 3.86, 4.05 | 19.56, 61.78 | ||
| plasmid+siRNA (scrambled) | .521, 19.31 | 2184, 2819 | 8705, 4133 | 32.29, 31.25 | 20.67, 603.95 | ||
| plasmid+siRNA (experimental) | 11.14, 354.3 | 1170, 1209 | 0.113, 0.114 | 15.58, 17.75 | 17.3, 182.4 |
W/F lab
| Sample | Green | Purple | Red | Blue | Pink | Yellow |
|---|---|---|---|---|---|---|
| No DNA | --. 0.042 | .048, .050 | ff 0.000,0.288 R 0.486,0.183 | ff 0.006,0.011 R 0.019,0.057 | ||
| plasmid only | 479.6, 206.2 | 2132, 17.75 | ff 53.18,60.96 R 138.1,66.05 | ff 2.276,40.13 R 10.41,131.7 | ||
| siRNA only | 0.055, 0.058 | .056, .013 | ff 0.047,0.117 R 0.241,0.299 | ff 0.011,0.012 R 0.024,0.140 | ||
| plasmid+siRNA (validated) | 210.6, 130.5 | 158.3, 0.963 | ff 57.02,57.56 R 28.75,13.65 | ff 5.476,5.378 R 12.73,28.36 | ||
| plasmid+siRNA (scrambled) | 1749, 1478 | 1167, 3.408 | ff 58.33,59.67 R 149.8,42.17 | ff 6.211,55.10 R 48.15,2427 | ||
| plasmid+siRNA (experimental) | 113, 107.1 | 202.9, 0.779 | ff 69.26,63.04 R 45.68,57.43 | ff 4.872,47.39 R 3.998,210.4 |
