Talk:20.109(F07): Luciferase assays and RNA prep: Difference between revisions

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Revision as of 10:23, 30 October 2007

Luciferase Assay Data

Refer to table from first day of module to know what part of the Renilla luciferase gene was targeted by each group.
Please list values for each of your duplicate samples, separated by a comma.
Please calculate out any dilutions that you may have performed on your lysates so that the values listed here reflect the measurements for undiluted samples.
If there were any unusual things that are worth noting about your experiment, please note them below the table for your day of lab and "sign" your comment with your team color.

T/R lab

Sample Green Purple Red Blue Pink Yellow
No DNA 0.0, .083 ff: 0.008, 0.009, R: 0.053, 0.054 394.3, 470.9 11.47, 9.00 ff: 0.012, 0.012; R: 0.049, 0.054 ff: 0.0103, 0.0031; R: 0.0903, 0.0616
plasmid only 11.12, 406.2 ff: 119.0, 109.3, R: 3826, 3299 5864, 9516 29.50, 30.04 ff: 21.42, 25.56; R: 618.0, 747.9 ff: 181.9, 195.1; R: 5175, 5496
siRNA only 0.009, .049 ff: 0.012, 0.013, R: .063, .051 354, 467.6 4.40, 10.40 ff: 0.007, 0.005; R: 0.064, 0.059 ff: 0.0068, 0.0257; R: 0.0866, 0.0721
plasmid+siRNA (validated) 23.21, 843.7 ff: 61.80, 86.20, R: 136.4, 177.7 0.058, 0.074 3.86, 4.05 ff: 29.88, 14.24; R: 81.25, 42.32 ff: 245.1, 255.8; R: 443.5, 438.5
plasmid+siRNA (scrambled) .521, 19.31 ff: 76.87, 88.13, R: 2184, 2819 8705, 4133 32.29, 31.25 ff: 26.32, 15.02; R: 760.3, 447.6 ff: 229.3, 220.0; R: 6976, 7308
plasmid+siRNA (experimental) 11.14, 354.3 ff: 124.2, 124.2, R: 1170, 1209 0.113, 0.114 15.58, 17.75 ff: 22.33, 12.16; R: 237.1, 127.7 ff: 223.1, 233.2; R: 748.7, 717.1

W/F lab

Sample Green Purple Red Blue Pink Yellow
No DNA --. 0.042 .048, .050 ff 0.059, 0.096; R 0.658, 1.164 ff 0.000,0.288 R 0.486,0.183 ff 0.006,0.011 R 0.019,0.057
plasmid only 479.6, 206.2 2132, 17.75 (1/10 dil) ff 9.332, 78.35; R 252.1, 1773 ff 53.18,60.96 R 138.1,66.05 ff 2.276,40.13 R 10.41,131.7
siRNA only 0.055, 0.058 .056, .013 ff 0.080, 0.120; R 0.481, 0.575 ff 0.047,0.117 R 0.241,0.299 ff 0.011,0.012 R 0.024,0.140
plasmid+siRNA (validated) 210.6, 130.5 158.3, 0.963 ff 781.6, 731.0; R 2114, 2537 ff 57.02,57.56 R 28.75,13.65 ff 5.476,5.378 R 12.73,28.36
plasmid+siRNA (scrambled) 1749, 1478 1167, 3.408 (1/10 dil) ff 162.2, 85.75; R 5424, 2225 ff 58.33,59.67 R 149.8,42.17 ff 6.211,55.10 R 48.15,2427
plasmid+siRNA (experimental) 113, 107.1 202.9, 0.779 ff 926.4, 644.1; R 2925, 1880 ff 69.26,63.04 R 45.68,57.43 ff 4.872,47.39 R 3.998,210.4
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