Talk:20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis

T/R lab

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. Nice work!!

And here's the gel with your recovered fragments/backbones, ready for ligation. Thanks to Michelle for running and posting this.

Lane	Sample	Lane	Sample
1	Blue Frag	8	Red BKB
2	Blue BKB	9	Orange Frag
3	Green Frag	10	Orange BKB
4	Green BKB	11	Purple Frag
5	Pink Frag	12	Purple BKB
6	Pink BKB	13	Yellow Frag
7	Red Frag	14	Yellow BKB

W/F lab

Drum roll.....Here's the W/F gel of the recovered products.
Green & Pink, you had a faint fragment band, but it was definitely there. Blue's band was very faint if there at all. (I increased the exposure to saturation, and thought I could see a band) Be sure to comment on these data in your lab report as well as possible sources of error. Even if you had perfect data, discussing possible areas for error is encouraged.

Use this image for your homework, but we have more fragment DNA if you need it.

Lane	Sample	Lane	Sample
1	Blue Frag	8	Yellow BKB
2	Blue BKB	9	Purple Frag
3	Green Frag	10	Purple BKB
4	Green BKB	11	Ladder
5	Pink Frag	12
6	Pink BKB	13
7	Yellow Frag	14

For the Green and Blue Teams, I re-ran a gel of your DNA this morning to double check. These samples were run according to the protocol in your lab book. (5 ul DNA) Assume that the ladder band you are interested in is still 125 ng. Since the intensities are different than we originally thought (my mistake), the ratio is off, but you ended up with viable colonies.

Lane	Sample
1	BKB
2	Ladder
3	Frag