Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture: Difference between revisions
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(New page: Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show...) |
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==T/R Sample Gel== | |||
Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours. | Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours. | ||
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==W/F Data== | |||
{| border="1" | |||
|Team colour | |||
|Digest enzyme 1 | |||
|Digest enzyme 2 | |||
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|Yellow | |||
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|Green | |||
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|Blue | |||
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|- | |||
|Pink | |||
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|- | |||
|Purple | |||
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|- | |||
|} | |||
[[Image:F08-M1D6_WF-Yellow.BMP|thumb|left|300px| W/F Yellow Group]] | |||
[[Image:F08-M1D6_WF-Green.BMP|thumb|left|300px| W/F Green Group]] | |||
[[Image:F08-M1D6_WF-Blue.BMP|thumb|left|300px| W/F Blue Group]] | |||
[[Image:F08-M1D6_WF-Pink.BMP|thumb|left|300px| W/F Pink Group]] | |||
[[Image:F08-M1D6_WF-Purple.BMP|thumb|left|300px| W/F Purple Group]] | |||
Revision as of 13:29, 26 September 2008
T/R Sample Gel
Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours.
| Lane | Plasmid | sample | volume to load |
|---|---|---|---|
| 1 | pCX-NNX | uncut | 20 μL |
| 2 | cut with BamHI | 20 μL | |
| 3 | cut with XhoI | 20 μL | |
| 4 | cut with BamHI and XhoI | 20 μL | |
| 5 | 1 kb marker | 10 μL | |
| 6 | candidate 1 | uncut | 20 μL |
| 7 | cut with BamHI | 20 μL | |
| 8 | cut with XhoI | 20 μL | |
| 9 | cut with BamHI and XhoI | 20 μL | |
| 10 | candidate 2 | uncut | 20 μL |
| 11 | cut with BamHI | 20 μL | |
| 12 | cut with XhoI | 20 μL | |
| 13 | cut with BamHI and XhoI | 20 μL |
W/F Data
| Team colour | Digest enzyme 1 | Digest enzyme 2 |
| Yellow | ||
| Green | ||
| Blue | ||
| Pink | ||
| Purple |
