Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture

T/R Sample Gel

Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours.

Lane	Plasmid	sample	volume to load
1	pCX-NNX	uncut	20 μL
2		cut with BamHI	20 μL
3		cut with XhoI	20 μL
4		cut with BamHI and XhoI	20 μL
5	1 kb marker		10 μL
6	candidate 1	uncut	20 μL
7		cut with BamHI	20 μL
8		cut with XhoI	20 μL
9		cut with BamHI and XhoI	20 μL
10	candidate 2	uncut	20 μL
11		cut with BamHI	20 μL
12		cut with XhoI	20 μL
13		cut with BamHI and XhoI	20 μL

W/F Data

Please fill in which two enzymes you used in the following table.

You can download your gel images below. If the images do not show up properly in your browser, just click on the place where the image is supposed to be, and it should be visible on a new page.

Please also see the T/R sample gel above if your gel experiment did not work as you had hoped - that is, if your bands are too faint to decipher, if one or both of your enzymes didn't cut at all, or if none of your clones gave the expected product. In this case, you should analyze both your own data and the sample data in your report.

Team colour	Digest enzyme 1	Digest enzyme 2
Yellow	BamHI	XhoI
Green
Blue
Pink
Purple

W/F Yellow Group

W/F Green Group

W/F Blue Group

W/F Pink Group

W/F Purple Group