Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture
From OpenWetWare
T/R Sample Gel
Here's a sample gel that was run with interpretable results. If your gel didn't work at all, or if none of your clones is right, then you can also include this image in your report to show your understanding of the experiment. The samples prepared exactly as you were directed to prepare yours.
Lane | Plasmid | sample | volume to load |
---|---|---|---|
1 | pCX-NNX | uncut | 20 μL |
2 | cut with BamHI | 20 μL | |
3 | cut with XhoI | 20 μL | |
4 | cut with BamHI and XhoI | 20 μL | |
5 | 1 kb marker | 10 μL | |
6 | candidate 1 | uncut | 20 μL |
7 | cut with BamHI | 20 μL | |
8 | cut with XhoI | 20 μL | |
9 | cut with BamHI and XhoI | 20 μL | |
10 | candidate 2 | uncut | 20 μL |
11 | cut with BamHI | 20 μL | |
12 | cut with XhoI | 20 μL | |
13 | cut with BamHI and XhoI | 20 μL |
W/F Data
Please fill in which two enzymes you used in the following table.
You can download your gel images below. If the images do not show up properly in your browser, just click on the place where the image is supposed to be, and it should be visible on a new page.
Please also see the T/R sample gel. above if your gel experiment did not work as you had hoped.
Team colour | Digest enzyme 1 | Digest enzyme 2 |
Yellow | ||
Green | ||
Blue | ||
Pink | ||
Purple |