Talk:20.109(F08): Mod 1 Day 8 FACS analysis
The data from your time at flow cytometry is linked below. A sample of a cell population is found here File:M1D8 Sample Population.pic. If you need any other images, please email me and I will get them to you on Monday.
To see the images, you can download them and then open them up, or just click on the file and wait for quicktime to open them. They are easily opened on a mac, but pc can open them via quicktime or other similar program.
T/R Results
The Data is collected in this file File:M1D8 Thursday Results.doc. If your data is unusable, please use Friday Yellow data. Their conditions are at the bottom of the page. The images provided are from Sample 1, 2, 3, 4, 5, 8, 11, and 14. If you need any others, please email me.
| Team Color | Image File |
| Blue | File:M1 D8 TR Blue Layout.pic |
| Green | File:M1 D8 TR Green Layout.pic |
| Orange | File:M1 D8 TR Orange Layout.pic |
| Pink | File:M1 D8 TR Pink Layout.pic |
| Purple | File:M1D8 TR Purple Layout.pic |
| Red | File:M1D8 TR Red Layout.pic |
| Yellow | File:M1D8 TR Yellow Layout.pic |
W/F Results
The collected results are in this file File:Friday Results.doc
The images provided are from Sample 1, 2, 3, 4, 5, 8, 11, and 14. If you need any others, please email me.
| team color | Download this File |
| Yellow | File:Friday Yellow Layout.jpg |
| Green | File:Friday Green Layout.jpg |
| Blue | File:Friday Blue Layout.jpg |
| Pink | File:Friday Pink Layout.jpg |
| Purple | File:Friday Purple Layout.jpg |
If your data is unusable because of low/no cell counts, please use Friday Yellow's data. Their conditions are below. You need to discuss why your data is unusable and what may have caused it. As YingFei pointed out, the data point for Yellow #12 was garbled in the translation. It should be 2.55 e-3. This is a lot lower than the other replicates for the condition.
Now you have to make a choice. We can't ignore data that just doesn't 'fit' our expectations. Is there sufficient reason to throw this data point out? (machine stopped working, killed all the cells, low cell count, low transfection) The machine worked fine, there will 40,000 cells, there was ~30% transfection. If you think there is a valid reason then explain it, otherwise, analyze as normal.
| Sample | Condition |
| 1 | Mock |
| 2 | EGFP |
| 3 | Delta 5 |
| 4 | Delta 3 |
| 5, 6, 7 | Delta 5 (.1 ug) + Delta 3 (.1 ug) |
| 8, 9, 10 | Delta 5 (.5 ug) + Blunt Delta 3 (.1 ug) |
| 11, 12, 13 | Delta 5 (.5 ug) + Delta 3 (.1 ug) |
| 14, 15, 16 | Delta 5 (.1 ug) + Blunt Delta 3 (.1 ug) |
