Talk:20.109(F09): Mod 1 Day 6 Restriction map and tissue culture

Part 1: Agarose Gel Electrophoresis

Your samples were run on a 1% agarose gel at 125 volts for 30-45 min. This is the order samples should have been loaded. If you did yours differently, please note under the image of your gel.

Lane	Plasmid	sample	volume to load
1	pCX-NNX	uncut	20 μL
2		cut with enzyme 1	20 μL
3		cut with enzyme 2	20 μL
4		cut with enzymes 1 and 2	20 μL
5	1 kb marker		10 μL
6	candidate 1	uncut	20 μL
7		cut with enzyme 1	20 μL
8		cut with enzyme 2	20 μL
9		cut with enzymes 1 and 2	20 μL
10	candidate 2	uncut	20 μL
11		cut with enzyme 1	20 μL
12		cut with enzyme 2	20 μL
13		cut with enzymes 1 and 2	20 μL
14	1 kb marker		10 μL

T/R lab

Below is an image of your gel of the diagnostic digest. Please fill in the enzymes you used and what bands you expected for your double digest

Red	Orange	Yellow	Green
Enzymes: Expected double digest bands: ####	Enzymes: Expected double digest bands: #####	Enzymes: BamHI, SalI Expected double digest bands: 3800, 1600	Enzymes: XhoI, EcoRV Expected double digest bands: 547bp, ~4797bp
Blue	Purple	Pink
Enzymes: BamHI & Xho1 Expected double digest bands: 1172bp & 4170 bp	Enzymes: BamHI & XhoI Expected double digest bands: 1184bp & 4160bp	Enzymes: BamHI & Xho1 Expected double digest bands: 1198bp & 4160bp

W/F lab

Below is an image of your gel of the diagnostic digest. Please fill in what enzymes you used and the bands you expected for your double digest

Red	Green	Blue	Purple
Enzymes: Xho1 and BamH1 Expected double digest bands: 3701 and 1629	Enzymes: BamH1 and xho1 Expected double digest bands: 1629 and 3701	Enzymes: BamHI and NotI Expected double digest bands: 1214 and 4132	Enzymes: Expected double digest bands:
Pink
Enzymes: BamH1 XhoI Expected double digest bands: 1629 and 3701