Talk:20.109(F10): Mod 2 Day 7 Readout of Protein: Difference between revisions
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Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)<br> | Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)<br> | ||
[[Image:Graph1.png]] | [[Image:Graph1.png]] | ||
==Western Blot Pink^2 (Shown Above)== | |||
Comparing Cph8 expression between NB334, NB188+pCph8, Mutant Candidate 1, Mutant Candidate 2 <br> | |||
Also comparing Cph8 expression between light and dark exposed cultures for each <br><br> | |||
From right to left <br> | |||
1 Kal Marker <br> | |||
2 H6EnvZ <br> | |||
3 NB334 Light <br> | |||
4 NB334 Dark <br> | |||
5 NB188+pCph8 Light <br> | |||
6 NB188+pCph8 Dark <br> | |||
7 Mutant 1 Light <br> | |||
8 Mutant 1 Dark <br> | |||
9 Matant 2 Light <br> | |||
10 Mutant 2 Dark <br> |
Latest revision as of 19:48, 9 November 2010
Western Blot Results
All team blots shown below (except red which chose not to develop theirs).
Each team will need to label their lanes.
Team Pink did a second Western with interesting results. They should add labels and a brief explanation of their work.
beta-gal Activity assay (Blue team)
Error bars denote 1 stdev from the mean. Experiments were performed in triplicate.
Mut1 has an MGG mutation
Mut2 is unsequenced, but is probably loss-of-function
Also, L and D were mistakenly swapped (L bars denote dark trials, D bars denote light trials)
Western Blot Pink^2 (Shown Above)
Comparing Cph8 expression between NB334, NB188+pCph8, Mutant Candidate 1, Mutant Candidate 2
Also comparing Cph8 expression between light and dark exposed cultures for each
From right to left
1 Kal Marker
2 H6EnvZ
3 NB334 Light
4 NB334 Dark
5 NB188+pCph8 Light
6 NB188+pCph8 Dark
7 Mutant 1 Light
8 Mutant 1 Dark
9 Matant 2 Light
10 Mutant 2 Dark