Talk:20.109(F11): Mod 1 Day 3 Agarose gel electrophoresis: Difference between revisions
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===Here are the pre-purification gels for reference=== | ===Here are the pre-purification gels for reference=== | ||
[[Image:F11_M1D3_WF-Blu|250px|thumb|right| Blue Group]] | [[Image:F11_M1D3_WF-Blu.jpg|250px|thumb|right| Blue Group]] | ||
[[Image:F11_M1D3_WF-Grn|250px|thumb|left| Green Group]] | [[Image:F11_M1D3_WF-Grn.jpg|250px|thumb|left| Green Group]] | ||
[[Image:F11_M1D3_WF-Org|250px|thumb|left| | [[Image:F11_M1D3_WF-Org.jpg|250px|thumb|center| Orange Group]] | ||
[[Image:F11_M1D3_WF-Pnk.jpg|250px|thumb|right| Pink Group]] | |||
[[Image:F11_M1D3_WF-Red.jpg|250px|thumb|left| Red Group]] | |||
[[Image:F11_M1D3_WF-Ylw.jpg|250px|thumb|center| Yellow Group]] | |||
Revision as of 13:01, 23 September 2011
Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your lab report if you so choose.
T/R lab
| Red | Orange | Yellow | Green |
| Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Tuesday/Thursday Gel 1:
Lane 1: Red Fragment
Lane 2: Red Backbone
Lane 3:Orange Fragment
Lane 4:Orange Backbone
Lane 5: Marker
Lane 6: Yellow Fragment
Lane 7:Yellow Backbone
Lane 8:Green Fragment
Lane 9:Green Backbone
Lane 10:Blank
Tuesday/Thursday Gel 2:
Lane 1: Blue Fragment
Lane 2: Blue Backbone
Lane 3:Pink Fragment
Lane 4:Pink Backbone
Lane 5: Marker
Lane 6: Purple Fragment
Lane 7:Purple Backbone
Lane 8-10:Blank
W/F Lab
| Red | Orange | Yellow | Green |
| Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Here are the pre-purification gels for reference
