Talk:20.109(F11): Mod 1 Day 7 FACS analysis: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| (10 intermediate revisions by the same user not shown) | |||
| Line 97: | Line 97: | ||
{| border="1" | {| border="1" | ||
|team color | |team color | ||
|FACS time block (I = 12:30-2PM, II = 1:30- | |FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) | ||
|mock data | |mock data | ||
|EGFP data | |EGFP data | ||
| Line 109: | Line 109: | ||
|Red | |Red | ||
|III = 2:30-4PM | |III = 2:30-4PM | ||
| | |0.00 | ||
| | |64.35 | ||
| | |0.01 | ||
| | |0.02 | ||
| | |0.01 | ||
| | |0.02 | ||
| | |0.02 | ||
| | |not done | ||
|- | |- | ||
|Orange | |Orange | ||
|II = 1:30-3PM | |II = 1:30-3PM | ||
| | |0.00 | ||
| | |wasn't saved :( | ||
| | |0.02 | ||
| | |0.00 | ||
| | |1.02 | ||
| | |4.74 | ||
| | |0.00 | ||
| | |not done | ||
|- | |- | ||
|Yellow | |Yellow | ||
|I = 12:30-2PM | |I = 12:30-2PM | ||
| | |0.00 | ||
| | |56.33 | ||
| | |0.00 | ||
| | |0.00 | ||
| | |2.14 | ||
| | |6.34 | ||
| | |6.50 | ||
| | |0.89 (2:1 Δ5:Δ3, 0.15 μg DNA) | ||
|- | |- | ||
|Green | |Green | ||
|II = 1:30-3PM | |II = 1:30-3PM | ||
| | |0.00 | ||
| | |48.05 | ||
| | |0.01 | ||
| | |0.00 | ||
| | |2.25 | ||
| | |5.47 | ||
| | |6.54 | ||
| | |4.34 (50:50 of each Δ3 cut type added, 0.20 μg DNA total) | ||
|- | |- | ||
|Blue | |Blue | ||
|I = 12:30-2PM | |I = 12:30-2PM | ||
| | |0.00 | ||
| | |17.99 | ||
| | |0.00 | ||
| | |0.00 | ||
| | |1.36 | ||
| | |8.55 | ||
| | |6.00 | ||
| | |1.40 (3:1 Δ5:Δ3, 0.20 μg DNA total) | ||
|- | |- | ||
|Pink | |Pink | ||
|III = 2:30-4PM | |III = 2:30-4PM | ||
| | |0.00 | ||
| | |52.67 | ||
| | |0.01 | ||
| | |0.00 | ||
| | |1.46 | ||
| | |4.39 | ||
| | |3.80 | ||
| | |not done | ||
|} | |} | ||
Latest revision as of 13:24, 7 October 2011
Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.
T/R FACS schedule
| team color | FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) | mock data | EGFP data | delta 5 data | delta 3 data | delta5+delta3 | delta5+delta3(Pme) | delta5+delta3(Bam) | extra experiment (specify below) |
| Red | I = 12:30-2PM | 0.00 | 50.51 | 0.00 | 0.00 | 1.91 | 4.98 | 3.89 | not done |
| Orange | III = 2:30-4PM | 0.00 | 35.48 | 0.00 | 0.01 | 1.11 | 1.98 | 1.86 | not done |
| Yellow | I = 12:30-2PM | 0.00 | 45.28 | 0.00 | 0.00 | 0.89 | 3.33 | 2.59 | Not done |
| Green | I = 12:30-2PM | 0.00 | 58.5 | 0.01 | 0.01 | 1.61 | 4.33 | 4.92 | ethidium bromide (100 ng/ml), 0.24 |
| Blue | II = 1:30-3PM | 0.00 | 54.41 | 0.00 | 0.00 | 1.38 | 4.01 | 3.54 | Not done |
| Pink | II = 1:30-3PM | 0.00 | 46.04 | 0.01 | 0.00 | 1.19 | 3.97 | 2.69 | Not done |
| Purple | II = 1:30-3PM | 0.00 | 18.14 | 0.00 | 0.02 | 1.32 | 0.49 | 1.06 | Not done |
W/F FACS schedule
| team color | FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM) | mock data | EGFP data | delta 5 data | delta 3 data | delta5+delta3 | delta5+delta3(Pme) | delta5+delta3(Bam) | extra experiment (specify below) |
| Red | III = 2:30-4PM | 0.00 | 64.35 | 0.01 | 0.02 | 0.01 | 0.02 | 0.02 | not done |
| Orange | II = 1:30-3PM | 0.00 | wasn't saved :( | 0.02 | 0.00 | 1.02 | 4.74 | 0.00 | not done |
| Yellow | I = 12:30-2PM | 0.00 | 56.33 | 0.00 | 0.00 | 2.14 | 6.34 | 6.50 | 0.89 (2:1 Δ5:Δ3, 0.15 μg DNA) |
| Green | II = 1:30-3PM | 0.00 | 48.05 | 0.01 | 0.00 | 2.25 | 5.47 | 6.54 | 4.34 (50:50 of each Δ3 cut type added, 0.20 μg DNA total) |
| Blue | I = 12:30-2PM | 0.00 | 17.99 | 0.00 | 0.00 | 1.36 | 8.55 | 6.00 | 1.40 (3:1 Δ5:Δ3, 0.20 μg DNA total) |
| Pink | III = 2:30-4PM | 0.00 | 52.67 | 0.01 | 0.00 | 1.46 | 4.39 | 3.80 | not done |
