Talk:20.109(F11): Mod 2 Day 6 Readouts of DNA, Protein

Mutant Summary Data

Please summarize your findings in the appropriate table below. Include your first name and last initial so people who want to know more about your data can contact you. For the purposes of this table, just say that B-gal decreased/increased in the dark/light (relative to wild type), rather than giving exact Miller units.

Group Color	Group Names	Mutant 1 sequence change(s)	Mutant 1 B-gal summary results	Mutant 2 sequence change(s)	Mutant 2 B-gal summary results
T/R_Red	Shelley & Laura	A -> T	lower than kinase dead in light and dark	no mutation	similar to WT in light, lower beta gal in dark
T/R_Orange
T/R_Yellow	Cara, Queenie, Hannah	Data inconclusive	decrease in light & dark	Ala --> Glu	very low B-gal activity in light & dark
T/R_Green	Jon, Michael	Unknown	Decreased B-gal activity in light, better contrast	Unknown	Low B-gal activity in both light & dark
T/R_Blue	E. Brown, R. Park		B-gal decrease in light and dark		B-gal decrease in light and dark
T/R_Pink	Colin Beckwitt, Jamal Elkhader	No Mutation	B-gal decrease in light and dark (dark and light activity identical)	A553->K553(GCG->AAG), I566->V566(ATT->GTT)	B-gal decrease in light and dark (dark activity higher than light)
T/R_Purple

Group Color	Group Names	Mutant 1 sequence change(s)	Mutant 1 B-gal summary results	Mutant 2 sequence change(s)	Mutant 2 B-gal summary results
W/F_Red	Brook W, Edgar M	frameshift via deletions	Similar B-gal activity under both conditions (higher activity than light wild type)	frameshift via insertion	Similar B-gal activity under both conditions (higher activity than light wild type)
W/F_Orange	Mariana D, Divya C	Ala --> Val (GCG --> GTG)	B-gal decreased in both light and dark (dark and light activity identical)	No mutation	B-gal decrease in light
W/F_Yellow	Robin Y, Brian D	A -> T	decreased in both light and dark (no difference)	A -> I	decreased in both light and dark (no difference)
W/F_Green
W/F_Blue	Yimin C, Dan G	Ala-->Ser	B-gal decreased in both dark/light	Ala-->Asn	B-gal decreased in both dark/light
W/F_Pink/Purple	Luis J, Dorma F, Michelle F,	Alanine to Lysine	B-gal decreased in the dark	Couldn't read, improper priming	B-gal decreased in the dark