Talk:20.109(F12): Mod 2 Day 5 Assessing re-tuned system
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T/R Mutant Miller Assay
Please enter the results of your B-gal assay and your mutation (after sequencing)
Team Color | Mutant 1 B-Gal Average (Dark) | Mutant 1 B-Gal Average (Light) | Mutant 1 T541X; X = | Mutant 2 B-Gal Average (Dark) | Mutant 2 B-Gal Average (Light) | Mutant 2 T541X; X = | Wild Type System B-Gal (Dark) | Wild Type System B-Gal (Light) | H243A Mutant B-Gal (Dark) | H243A Mutant B-Gal (Light) |
Red | 1324.65 | 1054.65 | Ala | 1268.2 | 1106.4 | Leu | 944.2 | 330.8 | 71.95 | 66 |
Orange | 1077.76 | 351.56 | 1228.68 | 458.33 | 1105.34 | 418.94 | 65.42 | 63.91 | ||
Yellow | 1552.5 | 494.95 | WT | 1711.65 | 458.85 | WT | 1065.9 | 389.2 | 107.9 | 102.0 |
Green | 1287.79 | 531.26 | N/A (WT) | 749.18 | 390.12 | N/A (WT) | 1133.14 | 359.73 | 52.38 | 49.77 |
Blue | 1149.27 | 244.80 | None | 1208.32 | 532.60 | None | 1573.52 | 537.10 | 91.79 | 80.99 |
Pink | 1597.37 | 528.91 | Gly | 1037.31 | 237.98 | WT | 1000.73 | 323.69 | 49.52 | 48.48 |
Purple | 1208.35 | 1018.45 | Leu | 1328.9 | 190.95 | WT | 2192.9 | 322.85 | 43.15 | 38.80 |
W/F Mutant Miller Assay
Please enter the results of your B-gal assay and your mutation (after sequencing)
Team Color | Mutant 1 B-Gal Average (Dark) | Mutant 1 B-Gal Average (Light) | Mutant 1 T541X; X = | Mutant 2 B-Gal Average (Dark) | Mutant 2 B-Gal Average (Light) | Mutant 2 T541X; X = | Wild Type System B-Gal (Dark) | Wild Type System B-Gal (Light) | H243A Mutant B-Gal (Dark) | H243A Mutant B-Gal (Light) |
Green | ||||||||||
Blue | 1160 | 349 | Gly | 1463 | 1019 | Asn | 1500 | 627 | 92 | 112 |
Pink | 2328.25 | 1928.66 | -- | -- | 1099.13 | 414.85 | 54.54 | 45.27 | ||
Purple | 1417.88 | 1409.76 | 1450.07 | 454.78 | 1099.13 | 414.85 | 54.54 | 45.27 |