Talk:20.109(F13): Difference between revisions
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(New page: ==COLD-PCR product gels== ===T/R Section=== Image:TR_Gel1.BMP Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21: *Top gel #1: *La...) |
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Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21: | Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21: | ||
*Top gel #1: | *Top gel #1: | ||
*Lane 1: 100 bp MW ladder | *Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder] | ||
*Lane 2-5: White | *Lane 2-5: White | ||
*Lane 6-9: Blue | *Lane 6-9: Blue | ||
| Line 13: | Line 13: | ||
*Bottom gel #1: | *Bottom gel #1: | ||
*Lane 1: 100 bp MW ladder | *Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder] | ||
*Lane 2-5: Orange | *Lane 2-5: Orange | ||
*Lane 6-9: Pink | *Lane 6-9: Pink | ||
| Line 21: | Line 21: | ||
[[Image:TR_Gel2.BMP]] | [[Image:TR_Gel2.BMP]] | ||
*Top gel #2: | *Top gel #2: | ||
*Lane 1: 100 bp MW ladder | *Lane 1: https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder] | ||
*Lane 2-5: Green | *Lane 2-5: Green | ||
*Lane 6-9: Purple | *Lane 6-9: Purple | ||
*Lane 10-13: Platinum | *Lane 10-13: Platinum | ||
Note: The T/R gel was completed before PCR purification. | |||
W/F section -- | ==W/F section== | ||
[[Image: WF_postpurification_gel.BMP]] | |||
Lanes are loaded the same for each team HCC-827 exon 19, SKOV3 exon 19, SKOV3 exon 21: | |||
*Top Gel: | |||
*Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder] | |||
*Lane 2-4: White | |||
*Lane 5-7: Blue | |||
*Lane 8-10: Red | |||
*Lane 11-13: Orange | |||
*Bottom Gel: | |||
*Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder] | |||
*Lane 2-4: Pink | |||
*Lane 5-7: Yellow | |||
*Lane 8-10: Green | |||
*Lane 11-13: Purple | |||
Note: The W/F gel was completed after PCR purification. | |||
Revision as of 09:54, 16 October 2013
COLD-PCR product gels
T/R Section
Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21:
- Top gel #1:
- Lane 1: 100 bp MW ladder
- Lane 2-5: White
- Lane 6-9: Blue
- Lane 10-13: Red
- Lane 14: Aneesh
- Bottom gel #1:
- Lane 1: 100 bp MW ladder
- Lane 2-5: Orange
- Lane 6-9: Pink
- Lane 10-13: Yellow
- Lane 14: Aneesh
- Top gel #2:
- Lane 1: https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
- Lane 2-5: Green
- Lane 6-9: Purple
- Lane 10-13: Platinum
Note: The T/R gel was completed before PCR purification.
W/F section
Lanes are loaded the same for each team HCC-827 exon 19, SKOV3 exon 19, SKOV3 exon 21:
- Top Gel:
- Lane 1: Low Molecular Weight ladder
- Lane 2-4: White
- Lane 5-7: Blue
- Lane 8-10: Red
- Lane 11-13: Orange
- Bottom Gel:
- Lane 1: Low Molecular Weight ladder
- Lane 2-4: Pink
- Lane 5-7: Yellow
- Lane 8-10: Green
- Lane 11-13: Purple
Note: The W/F gel was completed after PCR purification.
