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Line 1: |
| ==COLD-PCR product gels==
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| ===T/R Section===
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| [[Image:TR_Gel1.BMP]]
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| Lanes are loaded the same for each team HCC-827 exon 19, HCC-827 exon 21, SKOV3 exon 19, SKOV3 exon 21:
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| *Top gel #1:
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| *Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
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| *Lane 2-5: White
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| *Lane 6-9: Blue
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| *Lane 10-13: Red
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| *Lane 14: Aneesh
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| *Bottom gel #1:
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| *Lane 1: [https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
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| *Lane 2-5: Orange
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| *Lane 6-9: Pink
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| *Lane 10-13: Yellow
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| *Lane 14: Aneesh
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| [[Image:TR_Gel2.BMP]]
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| *Top gel #2:
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| *Lane 1: https://www.neb.com/products/n0467-quick-load-100-bp-dna-ladder 100 bp MW ladder]
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| *Lane 2-5: Green
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| *Lane 6-9: Purple
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| *Lane 10-13: Platinum
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| Note: The T/R gel was completed before PCR purification.
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| ==W/F section==
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| [[Image: WF_postpurification_gel.BMP]]
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| Lanes are loaded the same for each team HCC-827 exon 19, SKOV3 exon 19, SKOV3 exon 21:
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| *Top Gel:
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| *Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder]
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| *Lane 2-4: White
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| *Lane 5-7: Blue
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| *Lane 8-10: Red
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| *Lane 11-13: Orange
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| *Bottom Gel:
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| *Lane 1: [https://www.neb.com/products/n3233-low-molecular-weight-dna-ladder Low Molecular Weight ladder]
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| *Lane 2-4: Pink
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| *Lane 5-7: Yellow
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| *Lane 8-10: Green
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| *Lane 11-13: Purple
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| Note: The W/F gel was completed after PCR purification.
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