Talk:20.109(F13): Fall 2013 schedule: Difference between revisions
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< | <center>'''20.109 SYLLABUS'''</center> | ||
'''Lecture:''' T/R 11-12 (16-220) <br> | |||
'''Lab:''' T/R 1-5 or W/F 1-5 (56-322)<br> | |||
'''People:''' Instructor and student web pages may be found at the linked [[20.109(F13):People | '''People''']] page. | |||
Welcome to 20.109! For some of you this will be the first time in a research lab and for others it will not; either way, it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping, and publicly presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value. | |||
'''ASSIGNMENT OVERVIEW''' | |||
You will perform three series of experiments (hereafter “modules”) over the course of the semester. The modules differ in both conceptual and technical content, and in the ways that your learning will be assessed. Links to the experiments and assignments are provided below. | |||
Although your lab work will be done in pairs, <font color = CC0000>'''most assignments will be submitted individually (as summarized below) and should reflect your personal understanding.'''</font color> Please read the 20.109 [[20.109(F13):Statement on collaboration and integrity | '''statement on collaboration and integrity''']] for more detail about academic honesty in our class. You are encouraged to ask the teaching faculty any questions you have about what constitutes appropriate collaboration. | |||
*Individual assignments (60% of grade): all lab notebooks, quizzes, and homeworks (unless otherwise noted in directions); Module 1 methods section; Module 2 research article and journal club presentation. | |||
*Team assignments (40% of grade): Module 1 abstract and data summary (draft and revision), Module 3 data summary, and Module 3 research proposal. | |||
We appreciate that time management can be a difficult skill to develop, and that learning takes place on many time-scales. However, assignments turned in at wildly disparate times create additional logistical burdens for the teaching faculty. <font color= CC0000><b>Therefore, late work (both daily and culminating assignments) will be penalized 1/3 of a letter grade for each day late and will not be accepted after a week.</b></font color> We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the late policy clarification in the assignment descriptions. | |||
We will endeavor to provide equal access to subject 20.109 for students with disabilities, in accordance with the Americans with Disabilities Act (ADA) of 1990. Please see the teaching faculty as soon as possible regarding needed accommodations. | |||
'''SCHEDULE''' | |||
{| border=1px | {| border=1px | ||
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| [[20.109(F13):Lab tour | Orientation]] | | [[20.109(F13):Lab tour | Orientation]] | ||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
| 1 | | 1 | ||
| 1 | | 1 | ||
| Tue/Wed Sept 10/11 | | Tue/Wed Sept 10/11 | ||
| [[20.109(F13): Mod 1 Day 1 DNA engineering using PCR | DNA engineering using PCR]] | | [[20.109(F13): Mod 1 Day 1 DNA engineering using PCR | DNA engineering using PCR]] <br> lab practical quiz | ||
|-- | |-- | ||
| 1 | | 1 | ||
Line 29: | Line 51: | ||
| 3 | | 3 | ||
| Tue/Wed Sept 17/18 | | Tue/Wed Sept 17/18 | ||
| [[20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis| Agarose gel electrophoresis]] | | [[20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis| Agarose gel electrophoresis]] <br> lab quiz | ||
|-- | |-- | ||
| 1 | | 1 | ||
Line 38: | Line 60: | ||
| 1 | | 1 | ||
| 4 | | 4 | ||
| Tue/Wed Sept 24/25 '''No lecture today.''' | | Tue/Wed Sept 24/25, '''No lecture today.''' | ||
| [[20.109(F13): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]] | | [[20.109(F13): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]] | ||
|-- | |-- | ||
Line 49: | Line 71: | ||
| 6 | | 6 | ||
| Tue/Wed Oct 1/2 | | Tue/Wed Oct 1/2 | ||
| [[20.109(F13): Mod 1 Day 6 Lipofection and paper discussion| Lipofection and paper discussion]] lab quiz | | [[20.109(F13): Mod 1 Day 6 Lipofection and paper discussion| Lipofection and paper discussion]]<br> lab quiz | ||
|-- | |-- | ||
| 1 | | 1 | ||
| 7 | | 7 | ||
| Thu/Fri Oct 3/4 | | Thu/Fri Oct 3/4 | ||
| [[20.109(F13): Mod 1 Day 7 FACS analysis| FACS analysis]] | | [[20.109(F13): Mod 1 Day 7 FACS analysis| FACS analysis]] | ||
|-- | |-- | ||
| | | | ||
| | | | ||
| Monday Oct 7 | | Monday Oct 7 | ||
|<br><font color = CC0000><b>M1 Methods Due 5pm</b></font color><br> | |<br><font color = CC0000><b>M1 Methods Due 5pm</b></font color><br> | ||
|-- | |-- | ||
Line 70: | Line 86: | ||
| 1 | | 1 | ||
| Tue/Wed Oct 8/9 | | Tue/Wed Oct 8/9 | ||
| [[20.109(F13): Mod 2 Day 1 System Design| System Design]] | | [[20.109(F13): Mod 2 Day 1 System Design| System Design]] | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 2 | | 2 | ||
| Thu/Fri Oct 10/11 | | Thu/Fri Oct 10/11 | ||
| [[20.109(F13): Mod 2 Day 2 Mutation Analysis| Mutation Analysis]]<br><font color = CC0000><b>M1 Abstract and Data Summary Draft Due</b></font color><br> | |||
| [[20.109(F13): Mod 2 Day 2 Mutation Analysis| Mutation Analysis | |||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
! colspan="3" | Columbus Day Break Monday, Tues Oct 14, 15 | |||
! colspan=" | |||
| '''No lab Tues or Wed''' | | '''No lab Tues or Wed''' | ||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 3 | | 3 | ||
| Thu/Fri Oct 17/18 | | Thu/Fri Oct 17/18 | ||
| [[20.109(F13): Mod 2 Day 3 Prepare for LTS| Analysis & Planning 1]] | | [[20.109(F13): Mod 2 Day 3 Prepare for LTS| Analysis & Planning 1]] | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 4 | | 4 | ||
| Tue/Wed Oct 22/23 | | Tue/Wed Oct 22/23 | ||
| [[20.109(F13): Mod 2 Day 4 Phosphotyrosine Western Blot Analysis| Phosphotyrosine Western Blot Analysis]]<br>lab quiz | |||
| [[20.109(F13): Mod 2 Day 4 Phosphotyrosine Western Blot Analysis| Phosphotyrosine Western Blot Analysis | |||
|-- | |-- | ||
| 2 | | 2 | ||
| 5 | | 5 | ||
| Thu/Fri Oct 24/25 | | Thu/Fri Oct 24/25 | ||
| [[20.109(F13):Journal club I (Day5)| Journal club I]]<br> | | [[20.109(F13):Journal club I (Day5)| Journal club I]]<br> | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 6 | | 6 | ||
| Tue/Wed Oct 29/30 | | Tue/Wed Oct 29/30 | ||
| [[20.109(F13): Mod 2 Day 6 Analysis & Planning 2| Analysis & Planning 2]] <br><font color = CC0000><b>M1 Abstract and Data Summary Revision Due</b></font color> | |||
| [[20.109(F13): Mod 2 Day 6 Analysis & Planning 2| Analysis & Planning 2]] | |||
|-- | |-- | ||
| 2 | | 2 | ||
| 7 | | 7 | ||
| Thu/Fri Oct 31/Nov 1 | | Thu/Fri Oct 31/Nov 1 | ||
| [[20.109(F13): Mod 2 Day 7 HTS and Analysis| HTS and Analysis]] | | [[20.109(F13): Mod 2 Day 7 HTS and Analysis| HTS and Analysis]] | ||
|-- | |-- | ||
| 2 | | 2 | ||
| 8 | | 8 | ||
| Tue/Wed Nov 5/6 | | Tue/Wed Nov 5/6 | ||
| [[20.109(F13):Journal club II (Day8)| Journal club II]] <br> lab quiz | |||
| [[20.109(F13):Journal club II (Day8)| Journal club II]] | |||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
! colspan="3" | Veteran's Day Break Monday Nov 11 | |||
! colspan=" | |||
| '''No lab Thurs or Fri Nov 7/8. <br><font color = CC0000>M2 Report Due Monday, Nov 11th 5pm </font color> <br>''' | | '''No lab Thurs or Fri Nov 7/8. <br><font color = CC0000>M2 Report Due Monday, Nov 11th 5pm </font color> <br>''' | ||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 1 | | 1 | ||
| Tue/Wed Nov 12/13 | | Tue/Wed Nov 12/13 | ||
| [[20.109(F13): Mod 3 Day 1 Growth of phage materials| Growth of phage materials]] | | [[20.109(F13): Mod 3 Day 1 Growth of phage materials| Growth of phage materials]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 2 | | 2 | ||
| Thu/Fri Nov 14/15 | | Thu/Fri Nov 14/15 | ||
| [[20.109(F13): Mod 3 Day 2 Phage nanowires| Phage nanowires]] <br> lab quiz | |||
| [[20.109(F13): Mod 3 Day 2 Phage nanowires| Phage nanowires]] | |||
|-- | |-- | ||
| 3 | | 3 | ||
| 3 | | 3 | ||
| Tue/Wed Nov 19/20 | | Tue/Wed Nov 19/20 | ||
| [[20.109(F13): Mod 3 Day 3 TEM| TEM]] | | [[20.109(F13): Mod 3 Day 3 TEM| TEM]] | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 4 | | 4 | ||
| Thu/Fri Nov 21/22 | | Thu/Fri Nov 21/22 | ||
| [[20.109(F13): Mod 3 Day 4 Solar cell assembly| Solar cell assembly]] | | [[20.109(F13): Mod 3 Day 4 Solar cell assembly| Solar cell assembly]] | ||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
! colspan="3" | Thanksgiving Break Nov 28, 29 | |||
! colspan=" | |||
| '''Lecture: yes, on Tuesday Nov 26th<br> Lab: none all week!<br><font color = CC0000> M2 Revision due Wednesday, Nov 27th 5pm</font color>''' | | '''Lecture: yes, on Tuesday Nov 26th<br> Lab: none all week!<br><font color = CC0000> M2 Revision due Wednesday, Nov 27th 5pm</font color>''' | ||
|-- | |-- | ||
| colspan=" | | colspan="4" | | ||
|-- | |-- | ||
| 3 | | 3 | ||
| 5 | | 5 | ||
| Tue/Wed Dec 3/4 | | Tue/Wed Dec 3/4 | ||
| [[20.109(F13): Mod 3 Day 5 Solar cell testing| Solar cell testing]] <br> lab quiz | |||
| [[20.109(F13): Mod 3 Day 5 Solar cell testing| Solar cell testing]] | |||
|-- | |-- | ||
| 3 | | 3 | ||
| 6 | | 6 | ||
| Thu/Fri Dec 5/6 | | Thu/Fri Dec 5/6 | ||
| [[20.109(F13): Mod 3 Day 6 Wrap-up and data summary| Wrap-up & data summary]]<br><font color = CC0000><b>M3 Data Summary Due</b></font color> | |||
| [[20.109(F13): Mod 3 Day 6 Wrap-up and data summary| Wrap-up & data summary]] | |||
|-- | |-- | ||
| 3 | | 3 | ||
| 7 | | 7 | ||
| Tue/Wed Dec 10/11 | | Tue/Wed Dec 10/11, '''No lecture today.''' | ||
| [[20.109(F13): Mod 3 Day 7 Oral presentations| Research proposal presentations]] | | [[20.109(F13): Mod 3 Day 7 Oral presentations| Research proposal presentations]] | ||
|-- | |-- | ||
|} | |} | ||
</div> | </div> |
Latest revision as of 10:00, 6 December 2013
Lecture: T/R 11-12 (16-220)
Lab: T/R 1-5 or W/F 1-5 (56-322)
People: Instructor and student web pages may be found at the linked People page.
Welcome to 20.109! For some of you this will be the first time in a research lab and for others it will not; either way, it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping, and publicly presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
ASSIGNMENT OVERVIEW
You will perform three series of experiments (hereafter “modules”) over the course of the semester. The modules differ in both conceptual and technical content, and in the ways that your learning will be assessed. Links to the experiments and assignments are provided below.
Although your lab work will be done in pairs, most assignments will be submitted individually (as summarized below) and should reflect your personal understanding. Please read the 20.109 statement on collaboration and integrity for more detail about academic honesty in our class. You are encouraged to ask the teaching faculty any questions you have about what constitutes appropriate collaboration.
- Individual assignments (60% of grade): all lab notebooks, quizzes, and homeworks (unless otherwise noted in directions); Module 1 methods section; Module 2 research article and journal club presentation.
- Team assignments (40% of grade): Module 1 abstract and data summary (draft and revision), Module 3 data summary, and Module 3 research proposal.
We appreciate that time management can be a difficult skill to develop, and that learning takes place on many time-scales. However, assignments turned in at wildly disparate times create additional logistical burdens for the teaching faculty. Therefore, late work (both daily and culminating assignments) will be penalized 1/3 of a letter grade for each day late and will not be accepted after a week. We strongly recommend that you plan ahead and space out your work when possible. For assignments that may be revised, be sure to read the late policy clarification in the assignment descriptions.
We will endeavor to provide equal access to subject 20.109 for students with disabilities, in accordance with the Americans with Disabilities Act (ADA) of 1990. Please see the teaching faculty as soon as possible regarding needed accommodations.
SCHEDULE
MODULE | DAY | DATE | LAB |
N/A | 0 | Thu/Fri Sept 5/6 | Orientation |
1 | 1 | Tue/Wed Sept 10/11 | DNA engineering using PCR lab practical quiz |
1 | 2 | Thu/Fri Sept 12/13 | Clean and cut DNA |
1 | 3 | Tue/Wed Sept 17/18 | Agarose gel electrophoresis lab quiz |
1 | 3.5 | Thu Sept 19 | We'll have lecture but no lab. MIT Break Friday Sept 20 |
1 | 4 | Tue/Wed Sept 24/25, No lecture today. | DNA ligation and bacterial transformation |
1 | 5 | Thu/Fri Sept 26/27 | Examine candidate clones and tissue culture |
1 | 6 | Tue/Wed Oct 1/2 | Lipofection and paper discussion lab quiz |
1 | 7 | Thu/Fri Oct 3/4 | FACS analysis |
Monday Oct 7 | M1 Methods Due 5pm | ||
2 | 1 | Tue/Wed Oct 8/9 | System Design |
2 | 2 | Thu/Fri Oct 10/11 | Mutation Analysis M1 Abstract and Data Summary Draft Due |
Columbus Day Break Monday, Tues Oct 14, 15 | No lab Tues or Wed | ||
---|---|---|---|
2 | 3 | Thu/Fri Oct 17/18 | Analysis & Planning 1 |
2 | 4 | Tue/Wed Oct 22/23 | Phosphotyrosine Western Blot Analysis lab quiz |
2 | 5 | Thu/Fri Oct 24/25 | Journal club I |
2 | 6 | Tue/Wed Oct 29/30 | Analysis & Planning 2 M1 Abstract and Data Summary Revision Due |
2 | 7 | Thu/Fri Oct 31/Nov 1 | HTS and Analysis |
2 | 8 | Tue/Wed Nov 5/6 | Journal club II lab quiz |
Veteran's Day Break Monday Nov 11 | No lab Thurs or Fri Nov 7/8. M2 Report Due Monday, Nov 11th 5pm | ||
3 | 1 | Tue/Wed Nov 12/13 | Growth of phage materials |
3 | 2 | Thu/Fri Nov 14/15 | Phage nanowires lab quiz |
3 | 3 | Tue/Wed Nov 19/20 | TEM |
3 | 4 | Thu/Fri Nov 21/22 | Solar cell assembly |
Thanksgiving Break Nov 28, 29 | Lecture: yes, on Tuesday Nov 26th Lab: none all week! M2 Revision due Wednesday, Nov 27th 5pm | ||
3 | 5 | Tue/Wed Dec 3/4 | Solar cell testing lab quiz |
3 | 6 | Thu/Fri Dec 5/6 | Wrap-up & data summary M3 Data Summary Due |
3 | 7 | Tue/Wed Dec 10/11, No lecture today. | Research proposal presentations |