Talk:20.109(F13): Mod 1 Day 7 FACS analysis: Difference between revisions
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==Data Summary== | ==Data Summary== | ||
To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion. For samples done in duplicate, please separate the two values with a semi-colon, and in general don't get over-excited with the number of significant figures you report! For example, "3.0; 2.7" will suffice. | To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion. For samples done in duplicate, please separate the two values with a semi-colon, and in general don't get over-excited with the number of significant figures you report! For example, "3.0; 2.7" will suffice. Please describe the ratio test condition in the form "0.2 ug D5 and 0.05 ug CUT D3." | ||
===T/R=== | ===T/R=== | ||
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{| border="1" | {| border="1" | ||
|Team color | |Team color | ||
|% R2 gated mock | |% R2 gated mock | ||
|" EGFP | |" EGFP | ||
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|" D5 + cut D3 + DMSO | |" D5 + cut D3 + DMSO | ||
|" D5 + cut D3 + C401 | |" D5 + cut D3 + C401 | ||
| | |% R2 Ratio test | ||
|Ratio condition | |||
|- | |- | ||
| | | | ||
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|- | |- | ||
|} | |} | ||
If you did not collect the default amount of R1 cells (X) per sample, please list your cell count below: | |||
*Team Example: 5000 | |||
* | |||
===W/F=== | ===W/F=== | ||
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{| border="1" | {| border="1" | ||
|Team color | |Team color | ||
|% R2 gated mock | |% R2 gated mock | ||
|" EGFP | |" EGFP | ||
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|" D5 + cut D3 + DMSO | |" D5 + cut D3 + DMSO | ||
|" D5 + cut D3 + C401 | |" D5 + cut D3 + C401 | ||
| | |% R2 Ratio test | ||
|Ratio condition | |||
|- | |- | ||
| | | | ||
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|- | |- | ||
|} | |} | ||
If you did not collect the default amount of R1 cells (X) per sample, please list your cell count below: | |||
*Team Example: 5000 | |||
* | |||
Revision as of 07:32, 3 October 2013
Schedule
Sign up for a time to do flow cytometry in the table below. Please arrive at the time you choose and then expect to spend about 45 minutes in TC and another 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.
T/R
| Time block | Team 1 | Team 2 | Team 3 |
| I: 1-2:30 pm | Blue | Platinum | White |
| II: 2-3:30-pm | Purple | Yellow | Green |
| III: 3-4:30 pm | Orange | Pink | Red |
W/F
| Time block | Team 1 | Team 2 | (Team 3) |
| I: 1-2:30 pm | Red Team | Orange Team | White Team |
| II: 2-3:30-pm | Green team | Pink Team | Blue Team |
| III: 3-4:30 pm | Purple Team |
Data Summary
To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion. For samples done in duplicate, please separate the two values with a semi-colon, and in general don't get over-excited with the number of significant figures you report! For example, "3.0; 2.7" will suffice. Please describe the ratio test condition in the form "0.2 ug D5 and 0.05 ug CUT D3."
T/R
| Team color | % R2 gated mock | " EGFP | " EGFP + DMSO | " delta 5 (D5) | " delta 3 (D3) | " D5 + D3 | " D5 + cut D3 | " D5 + cut D3 + DMSO | " D5 + cut D3 + C401 | % R2 Ratio test | Ratio condition |
If you did not collect the default amount of R1 cells (X) per sample, please list your cell count below:
- Team Example: 5000
W/F
| Team color | % R2 gated mock | " EGFP | " EGFP + DMSO | " delta 5 (D5) | " delta 3 (D3) | " D5 + D3 | " D5 + cut D3 | " D5 + cut D3 + DMSO | " D5 + cut D3 + C401 | % R2 Ratio test | Ratio condition |
If you did not collect the default amount of R1 cells (X) per sample, please list your cell count below:
- Team Example: 5000
