Talk:20.109(F13): Mod 2 Day 6 Analysis & Planning 2: Difference between revisions
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===TR Blots=== | ===TR Blots=== | ||
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]] | '''TR Intensities''' | ||
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]] | Defaults | ||
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]] | *EGFR - 700:5, 800:3 | ||
*GAPDH - 700:2, 800:5 | |||
*Akt - 700:2, 800: 6 | |||
*ERK - 700:1, 800:3 | |||
*Pink STAT3 - 700:7, 800:9 | |||
*Pink EGFR - 700:7, 800:3 | |||
[[Image:Yellow_TR_EGFR.tif|thumb|left|300px|Yellow EGFR]] | |||
[[Image:Yellow_TR_ERK.tif|thumb|left|300px|Yellow ERK]] | |||
[[Image:Purple_TR_EGFR.tif|thumb|left|300px|Purple EGFR]] | |||
[[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]] | [[Image:Purple_TR_ERK.tif|thumb|left|300px|Purple ERK]] | ||
[[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]] | [[Image:White_TR_EGFR.tif|thumb|left|300px|White EGFR]] | ||
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[[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]] | [[Image:Green_TR_pAkt.tif|thumb|left|300px|Green pAkt]] | ||
[[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]] | [[Image:Green_TR_GAPDH.tif|thumb|left|300px|Green GAPDH]] | ||
[[Image:Green_TR_pAkt2.tif|thumb|left|300px|Green pAkt_2]] | |||
[[Image:Green_TR_GAPDH2.tif|thumb|left|300px|Green GAPDH_2]] | |||
[[Image:Green_TR_totalAkt2.tif|thumb|left|300px|Green Total Akt 2]] | |||
[[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]] | [[Image:Orange_TR_EGFR.tif|thumb|left|300px|Orange EGFR]] | ||
[[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]] | [[Image:Orange_pAkt.tif|thumb|left|300px|Orange pAkt]] | ||
[[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]] | [[Image:Orange_TR_GAPDH.tif|thumb|left|300px|Orange GAPDH]] | ||
[[Image:Orange_TR_pAkt2.tif|thumb|left|300px|Orange pAkt_2]] | |||
[[Image:Orange_TR_GAPDH2.tif|thumb|left|300px|Orange GAPDH_2]] | |||
[[Image:Orange_TR_totalAkt2.tif|thumb|left|300px|Orange Total Akt 2]] | |||
[[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]] | [[Image:Pink_TR_EGFR2.tif|thumb|left|300px|Pink EGFR]] | ||
[[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]] | [[Image:Pink_TR_STAT3.tif|thumb|left|300px|Pink STAT3]] | ||
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[[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]] | [[Image:Red_TR_STAT3.tif|thumb|left|300px|Red STAT3]] | ||
[[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]] | [[Image:Red_TR_GAPDH.tif|thumb|left|300px|Red GAPDH]] | ||
[[Image:Green_EGFR.tif|thumb|left|300px|Green EGFR]] | |||
[[Image:Green_pAkt.tif|thumb|left|300px|Green pAkt]] | |||
[[Image:Green_GAPDH.tif|thumb|left|300px|Green GAPDH]] | |||
[[Image:Plat_EGFR.tif|thumb|left|300px|Plat EGFR]] | |||
[[Image:Plat_pAkt.tif|thumb|left|300px|Plat pAkt]] | |||
[[Image:Plat_GAPDH.tif|thumb|left|300px|Plat GAPDH]] | |||
[[Image:Plat_TR_pAkt2.tif|thumb|left|300px|Plat pAkt_2]] | |||
[[Image:Plat_TR_GAPDH2.tif|thumb|left|300px|Plat GAPDH_2]] | |||
[[Image:Plat_TR_totalAkt2.tif|thumb|left|300px|Plat Total Akt 2]] | |||
<br style="clear:both;"/> | |||
=== | ===WF Blots=== | ||
* | '''WF Intensities''' | ||
ERK | |||
*White: 2/3 | |||
*Pink: 1.5/3 | |||
EGFR: | *Green: 2.5/3 | ||
STAT3 | |||
*Red: 6/7.5 | |||
*Blue: 6/8 | |||
GAPDH: | GAPDH (All Teams) | ||
* 2/5 | |||
[[Image:Yellow_WF_EGFR.tif|thumb|center|300px|Yellow EGFR]] | |||
[[Image:Yellow_WF_pAkt.tif|thumb|center|300px|Yellow pAkt]] | |||
Pink EGFR: | [[Image:Yellow_WF_GAPDH.tif|thumb|center|300px|Yellow GAPDH]] | ||
[[Image:Yellow_WF_totalAkt.tif|thumb|center|300px|Yellow total Akt]] | |||
*Note, Yellow blots are in the reverse order. | |||
[[Image:Purple_WF_EGFR.tif|thumb|center|300px|Purple EGFR]] | |||
[[Image:Purple_pAkt.tif|thumb|center|300px|Purple pAkt]] | |||
[[Image:Purple_WF_GAPDH.tif|thumb|center|300px|Purple GAPDH]] | |||
[[Image:Orange_WF_EGFR.tif|thumb|center|300px|Orange EGFR]] | |||
[[Image:Orange_WF_GAPDH.tif|thumb|center|300px|Orange GAPDH]] | |||
[[Image:Red_WF_EGFR.tif|thumb|center|300px|Red EGFR]] | |||
[[Image:Red_WF_STAT3_2.tif|thumb|center|300px|Red STAT3]] | |||
[[Image:Red_WF_GAPDH.tif|thumb|center|300px|Red GAPDH]] | |||
[[Image:Blue_WF_EGFR.tif|thumb|center|300px|Blue EGFR]] | |||
[[Image:Blue_WF_STAT3_2.tif|thumb|center|300px|Blue STAT3]] | |||
[[Image:Blue_WF_GAPDH.tif|thumb|center|300px|Blue GAPDH]] | |||
[[Image:Pink_WF_EGFR.tif|thumb|center|300px|Pink EGFR]] | |||
[[Image:Pink_WF_ERK.tif|thumb|center|300px|Pink ERK]] | |||
[[Image:Green_WF_EGFR.tif|thumb|center|300px|Green EGFR]] | |||
[[Image:Green_WF_ERK.tif|thumb|center|300px|Green ERK]] | |||
[[Image:White_WF_EGFR.tif|thumb|center|300px|White EGFR]] | |||
[[Image:White_WF_Erk.tif|thumb|center|300px|White ERK]] |
Latest revision as of 09:17, 6 November 2013
TR Blots
TR Intensities Defaults
- EGFR - 700:5, 800:3
- GAPDH - 700:2, 800:5
- Akt - 700:2, 800: 6
- ERK - 700:1, 800:3
- Pink STAT3 - 700:7, 800:9
- Pink EGFR - 700:7, 800:3
WF Blots
WF Intensities ERK
- White: 2/3
- Pink: 1.5/3
- Green: 2.5/3
STAT3
- Red: 6/7.5
- Blue: 6/8
GAPDH (All Teams)
- 2/5
- Note, Yellow blots are in the reverse order.