Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions

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===Diagnostic Digests===
==Colony Counts==


===W/F lab===


*'''Gel Pattern:'''
Please put your colony count data in the correct row.
 
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
 
{| border="1"
! Group Colour
! pCX-EGFP (#)
! bkb + ins, no lig (#)
! bkb + lig, no ins (#)
! bkb + ins, lig 1 (#)
! bkb + ins, lig 2 (#)
|-
| Hypothetical Data
| 1000
| 2
| 20
| 100
| 100
|-
|Blue
|1600
|0
|n/a
|0
|0
|-
|Green
|Lawn
|0
|4
|13
|14
|-
|Pinkle
|Lawn
|2
|2
|210
|305
|-
|}
 
<br style="clear:both;"/>
 
==Diagnostic Digests==
 
==='''Gel Pattern:'''===
   
   
Colony 1: Uncut, RE-1, RE-2, Double Digest
1st Colony: Uncut, RE-1, RE-2, Double Digest


Colony 2: Uncut, RE-1
2nd Colony: Uncut, RE-1


1kb Ladder, NEB
1kb Ladder, NEB


Colony 2: RE-2, Double Digest
2nd Colony: RE-2, Double Digest


pCX-NNX: Uncut, RE-1, RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
Line 16: Line 63:
1kb Ladder, NEB
1kb Ladder, NEB


==T/R lab==
===T/R lab===
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).


*Red Group:
[[Image:TR_Red_DD20109_F14.bmp|thumb|350px|right|Red Group: RE-1: B ; RE-2: P]]
[[Image:TR_Red_DD20109_F14.bmp]]
 
RE-1: B ; RE-2: P
[[Image:TR_Orange_DD20109_F14.bmp|thumb|350px|left|Orange Group: RE-1: BamHI ; RE-2: PstI]]
 
[[Image:TR_Yellow_DD20109_F14.bmp|thumb|350px|right|Yellow Group: RE-1: B ; RE-2: N]]
 
[[Image:TR_Green_DD20109_F14.bmp|thumb|350px|left|Green Group: RE-1: PvuI ; RE-2: EcoRV]]
 
[[Image:TR_Blue_DD20109_F14.bmp|thumb|350px|right| Blue Group: RE-1: BamHI ; RE-2: SalI]]
 
[[Image:TR_Pink_DD20109_F14.bmp|thumb|350px|left|Pink Group: RE-1: BamHI ; RE-2: PvuI]]
 
[[Image:TR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E_1 ; RE-2: E_2]]
<br style="clear:both;"/>
 
===W/F lab===


*Orange Group:
[[Image:WR_Green_low_DD20109_F14.bmp|thumb|350px|left|Green Group, low exposure: RE-1: Bam ; RE-2: Ska]]
[[Image:TR_Orange_DD20109_F14.bmp]]
RE-1: BamHI ; RE-2: PstI


*Yellow Group:
[[Image:WR_Green_high_DD20109_F14.bmp|thumb|350px|right|Green Group, high exposure: RE-1: Bam; RE-2: Ska ]]
[[Image:TR_Yellow_DD20109_F14.bmp]]
RE-1: B ; RE-2: N


*Green Group:
[[Image:WR_Blue_low_DD20109_F14.bmp|thumb|350px|left|Blue Group, low exposure: RE-1: EcoRV ; RE-2: PvuI ]]
[[Image:TR_Green_DD20109_F14.bmp]]
RE-1: PvuI ; RE-2: EcoRV


*Blue Group:
[[Image:WR_Blue_high_DD20109_F14.bmp|thumb|350px|right|Blue Group, high exposure: RE-1: EcoRV  ; RE-2: PvuI ]]
[[Image:TR_Blue_DD20109_F14.bmp]]
RE-1: BamHI ; RE-2: SalI


*Pink Group:
[[Image:WR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E1 ; RE-2: E2]]
[[Image:TR_Pink_DD20109_F14.bmp]]
RE-1: BamHI ; RE-2: PvuI


*Purple Group:
<br style="clear:both;"/>
[[Image:TR_Purple_DD20109_F14.bmp]]
RE-1: E_1 ; RE-2: E_2

Latest revision as of 18:24, 29 September 2014

Colony Counts

W/F lab

Please put your colony count data in the correct row.

Note: "hypothetical data" just shows made-up numbers similar to what you might expect.

Group Colour pCX-EGFP (#) bkb + ins, no lig (#) bkb + lig, no ins (#) bkb + ins, lig 1 (#) bkb + ins, lig 2 (#)
Hypothetical Data 1000 2 20 100 100
Blue 1600 0 n/a 0 0
Green Lawn 0 4 13 14
Pinkle Lawn 2 2 210 305


Diagnostic Digests

Gel Pattern:

1st Colony: Uncut, RE-1, RE-2, Double Digest

2nd Colony: Uncut, RE-1

1kb Ladder, NEB

2nd Colony: RE-2, Double Digest

pCX-NNX: Uncut, RE-1, RE-2, Double Digest

1kb Ladder, NEB

T/R lab

Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).

Red Group: RE-1: B ; RE-2: P
Orange Group: RE-1: BamHI ; RE-2: PstI
Yellow Group: RE-1: B ; RE-2: N
Green Group: RE-1: PvuI ; RE-2: EcoRV
Blue Group: RE-1: BamHI ; RE-2: SalI
Pink Group: RE-1: BamHI ; RE-2: PvuI
Purple Group: RE-1: E_1 ; RE-2: E_2


W/F lab

Green Group, low exposure: RE-1: Bam ; RE-2: Ska
Green Group, high exposure: RE-1: Bam; RE-2: Ska
Blue Group, low exposure: RE-1: EcoRV ; RE-2: PvuI
Blue Group, high exposure: RE-1: EcoRV  ; RE-2: PvuI
Purple Group: RE-1: E1 ; RE-2: E2


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