Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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Colony 2: Uncut, RE-1 | Colony 2: Uncut, RE-1 | ||
1kb Ladder | 1kb Ladder, NEB | ||
Colony 2: RE-2, Double Digest | Colony 2: RE-2, Double Digest | ||
| Line 14: | Line 14: | ||
Colony 3: Uncut, RE-1, RE-2, Double Digest | Colony 3: Uncut, RE-1, RE-2, Double Digest | ||
1kb Ladder, NEB | |||
==T/R lab== | ==T/R lab== | ||
Revision as of 11:10, 26 September 2014
Diagnostic Digests
- Gel Pattern:
Colony 1: Uncut, RE-1, RE-2, Double Digest
Colony 2: Uncut, RE-1
1kb Ladder, NEB
Colony 2: RE-2, Double Digest
Colony 3: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
- Red Group:
RE-1: B ; RE-2: P
- Orange Group:
RE-1: BamHI ; RE-2: PstI
- Yellow Group:
RE-1: B ; RE-2: N
- Green Group:
RE-1: PvuI ; RE-2: EcoRV
- Blue Group:
RE-1: BamHI ; RE-2: SalI
- Pink Group:
RE-1: BamHI ; RE-2: PvuI
- Purple Group:
RE-1: E_1 ; RE-2: E_2
