Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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[[Image:TR_Green_DD20109_F14.bmp|thumb|350px|left|Green Group: RE-1: PvuI ; RE-2: EcoRV]] | [[Image:TR_Green_DD20109_F14.bmp|thumb|350px|left|Green Group: RE-1: PvuI ; RE-2: EcoRV]] | ||
[[Image:TR_Blue_DD20109_F14.bmp|thumb|350px|right| Blue Group: RE-1: BamHI ; RE-2: SalI]] | |||
[[Image:TR_Blue_DD20109_F14.bmp | |||
RE-1: BamHI ; RE-2: SalI | |||
[[Image:TR_Pink_DD20109_F14.bmp|thumb|350px|left|Pink Group: RE-1: BamHI ; RE-2: PvuI]] | |||
[[Image:TR_Pink_DD20109_F14.bmp | |||
RE-1: BamHI ; RE-2: PvuI | |||
[[Image:TR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E_1 ; RE-2: E_2]] | |||
[[Image:TR_Purple_DD20109_F14.bmp | <br style="clear:both;"/> | ||
RE-1: E_1 ; RE-2: E_2 | |||
===W/F lab=== | ===W/F lab=== | ||
Revision as of 13:04, 26 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
W/F lab
| Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
|---|---|---|---|---|---|
| Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |
