Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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Revision as of 00:13, 29 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
---|---|---|---|---|---|
Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |
Blue | 1600 | 0 | n/a | 0 | 0 |
Green | Lawn | 0 | 4 | 13 | 14 |
Pinkle | Lawn | 2 | 2 | 210 | 305 |
Yellow Mustard | Lawn | 27 | 55 | Lawn | Lawn |
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
W/F lab