Talk:20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture: Difference between revisions
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===W/F lab=== | ===W/F lab=== | ||
[[Image:WR_Green_low_DD20109_F14.bmp|thumb|350px|left|Green Group, low exposure: RE-1: Bam ; RE-2: Ska]] | |||
[[Image:WR_Green_high_DD20109_F14.bmp|thumb|350px|right|Green Group, high exposure: RE-1: Bam; RE-2: Ska ]] | |||
[[Image:WR_Blue_low_DD20109_F14.bmp|thumb|350px|left|Blue Group, low exposure: RE-1: EcoRV ; RE-2: PvuI ]] | |||
[[Image:WR_Blue_high_DD20109_F14.bmp|thumb|350px|right|Blue Group, high exposure: RE-1: EcoRV ; RE-2: PvuI ]] | |||
[[Image:WR_Purple_DD20109_F14.bmp|thumb|350px|left|Purple Group: RE-1: E1 ; RE-2: E2]] | |||
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Latest revision as of 18:24, 29 September 2014
Colony Counts
W/F lab
Please put your colony count data in the correct row.
Note: "hypothetical data" just shows made-up numbers similar to what you might expect.
| Group Colour | pCX-EGFP (#) | bkb + ins, no lig (#) | bkb + lig, no ins (#) | bkb + ins, lig 1 (#) | bkb + ins, lig 2 (#) |
|---|---|---|---|---|---|
| Hypothetical Data | 1000 | 2 | 20 | 100 | 100 |
| Blue | 1600 | 0 | n/a | 0 | 0 |
| Green | Lawn | 0 | 4 | 13 | 14 |
| Pinkle | Lawn | 2 | 2 | 210 | 305 |
Diagnostic Digests
Gel Pattern:
1st Colony: Uncut, RE-1, RE-2, Double Digest
2nd Colony: Uncut, RE-1
1kb Ladder, NEB
2nd Colony: RE-2, Double Digest
pCX-NNX: Uncut, RE-1, RE-2, Double Digest
1kb Ladder, NEB
T/R lab
Here you find your gels from the diagnostic digest. On the side of each gel is written, what RE-1 and RE-2 is for your group (according to your tube labeling).
W/F lab
