Talk:20.109(S07): Bio-material engineering/Sequence analysis: Difference between revisions
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|Blue | |Blue | ||
| | |C | ||
| | |YALQA SGGGG SGGGG SGGGG SASQG GGGSG RMXD* TAXSN YF*LX RPV AXXRI RTKAY F*RGL VIARD LITTX XM* | ||
| | |weaker than pAu1 | ||
| | |sequence was uncertain. There were no conserved amino acids. The sequence also has closely spaced stop codons compared to pAu1 | ||
|- | |- | ||
|Blue | |||
|D | |||
|YALQA SGGGG SGGGG SGGGX SASQG GGGSX XRGXR RXXEX GS*LX RPVAX XRIRT KAYF* RGLVI ARDLI TTXXM* | |||
|weaker than pAu1, but stronger than candidate C. There are some conserved amino acids with pAu1. This might account for it being a better gold binder than candidate C. | |||
|sequence is very uncertain. | |||
|Pink | |Pink | ||
|A | |A |
Revision as of 11:06, 15 May 2007
Tuesday/Thursday Lab Sequence Data
team color | candidate number | sequence | relative strength | comments/notes | |||||
Green | A | XXXXXYXXXXXXXXXSGGXXSGGGXSAXQGXXXXGXXXXXXXXRXXXXXAXPXXXXXXXTXAYFXXXLXIXXXXXXTXXXXXXXXXSXXVFXDNS
STIEGXXPXXXPXXALXXXXXXXXXXXXXXXXXXAXXXDXXXGXXXXXXXGXXXXXXXXXXAXXXXXTKXXXXXXXXXXXXXXXXXXXXXXXXXXX |
as strong as pAu1 | too many unknown codons to analyze | |||||
Green | D | XXXXXYALQASGGGGSGGGGSGGGGSASQGGGGSGTRMRDAWXLMKGYLARPVAXXRIRTKAYF*RGLVIARDLITTXXM*XXXLXXXXVFXDN
XSXXXXXXXXKTXXTXXXY*VXXXXNSLXXXXXGXXXXR*XXXRRXFXXW*GDLXXXXXQFLXXXXXXNXXXXXTXXXLXLX*XXMXXNSIXYXXX |
as strong as pAu1 | seems as if the first stop codon may have mutated into a tyrosine, thus giving a longer gold-binding sequence | |||||
Purple | D | SGGGG SGGGG SGGGA SEIVL TQSPA LLSLS PGXRA TLSCR TSQSI SSTYL AWYQQ KPGQA PRLLI YGTSG RXTGI PYRFQ WXGSG XDFTX TISRL EPEDF AVXYC QQXAT SPRLL XGDQG GDQIR NSRTK AYF*R RLXXX XAASR SDTXC XC | weaker than pAU1 | a few of the amino acids are conserved although the order is not. our sequence is longer than that of pAU1 | |||||
Yellow | B | YALQA SGGGG SGGGG SGGGG SASQG GGXXX XXLXR XCXXL XXYXA RPVAX XRIRT KAYF* | weaker than pAu1 | most of amino acids in variable region are uncertain | |||||
Yellow | D | YALQA SGGGG SGGGG SGGGG SASQG GGGXX LXLPT XCTXX GXXLA RXVAX XRIRT KAYF* | weaker than pAu1 | sequence also fairly uncertain; no conserved amino acids observed, however, through trend of hydrophobic residues maintained | |||||
Blue | C | YALQA SGGGG SGGGG SGGGG SASQG GGGSG RMXD* TAXSN YF*LX RPV AXXRI RTKAY F*RGL VIARD LITTX XM* | weaker than pAu1 | sequence was uncertain. There were no conserved amino acids. The sequence also has closely spaced stop codons compared to pAu1 | |||||
Blue | D | YALQA SGGGG SGGGG SGGGX SASQG GGGSX XRGXR RXXEX GS*LX RPVAX XRIRT KAYF* RGLVI ARDLI TTXXM* | weaker than pAu1, but stronger than candidate C. There are some conserved amino acids with pAu1. This might account for it being a better gold binder than candidate C. | sequence is very uncertain. | Pink | A | YAXQA SGGGG SGGGG SGGGG SASQG GGGSG SRXXX SXLYH FLXLA XPVAX XRIRT KAYF* RGLVI XXDLI TXXXM* | weaker binder than pAU1 | presence of many uncertain amino acids |
Pink | C | YALQA SGGGG SGGGG SGGGG SASQG GGGSG GRWIR CWQXX PXXLA RPVAX XRIRT KAYF* | weaker binder than pAU1 | presence of many uncertain amino acids. Compared to candidate A, more amino acids match up with pAu1, better gold binder than candidate A. | |||||
Red | D | YALQA SGGGG SGGGG SGGGG SASQG GGGSG ERXLA CXKKF EWYLX RPVAX XRIRT KAYF | not really a gold binder (less colonies than pCT-CON) | Colony C sequencing failed, two glutamates in variable region |
Wednesday/Friday Lab Sequence Data
team color | candidate number | sequence | relative strength | comments/notes |
Green | ||||
Purple | ||||
Yellow | ||||
Blue | ||||
Pink | ||||
Red |
HAVE A GREAT SUMMER!!