Talk:20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6): Difference between revisions
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===TR lab=== | ===TR lab=== | ||
Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - | Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps. | ||
[[Image:S08_M1D6_TR-PiYR.jpg|thumb|right|300px| | [[Image:S08_M1D6_TR-PiYR.jpg|thumb|right|300px| Tue/Thu gels, 3 of 3]] | ||
[[Image:S08_M1D6_TR_PG.jpg|thumb|left|300px| | [[Image:S08_M1D6_TR_PG.jpg|thumb|left|300px| Tue/Thu gels, 1 of 3]] | ||
[[Image:S08_M1D6_TR-B.jpg|thumb|left|300px| | [[Image:S08_M1D6_TR-B.jpg|thumb|left|300px| Tue/Thu gels, 2 of 3]] | ||
{| border="1" | {| border="1" | ||
| Line 15: | Line 15: | ||
|- | |- | ||
|Green | |Green | ||
| | |XhoI+EcoRV | ||
| | |BamHI+SalI | ||
|- | |- | ||
|Purple | |Purple | ||
| Line 22: | Line 22: | ||
| | | | ||
|- | |- | ||
| Red | |Red | ||
| | |EcoRV+NotI | ||
| | |BamHI+XhoI | ||
|- | |- | ||
|Pink | |Pink | ||
| | |SalI+EcoRV | ||
| | |NotI+EcoRV | ||
|- | |- | ||
|Blue | |Blue | ||
| | | NotI+EcoRV | ||
| | | XhoI+BamHI | ||
|- | |- | ||
|Yellow | |Yellow | ||
| | |BamH1+Sal1 | ||
| | |EcoRV+Nsi1 | ||
|} | |} | ||
| Line 51: | Line 51: | ||
|- | |- | ||
|Green | |Green | ||
| | | XbaI/XhoI | ||
| | | BamHI/HsiI | ||
|- | |- | ||
|Purple | |Purple | ||
| Line 59: | Line 59: | ||
|- | |- | ||
| Red | | Red | ||
| | |BamHI/XhoI | ||
| | |BamHI/EcoRV | ||
|- | |- | ||
|Pink | |Pink | ||
| | |BamHI/SalI | ||
| | |BamHI/NsiI | ||
|- | |- | ||
|Blue | |Blue | ||
| | |BamHI/XhoI | ||
| | |Nsi1/EcoRV | ||
|- | |- | ||
|Yellow | |Yellow | ||
| | |XbaI/XhoI | ||
| | |BamHI/Nsil | ||
|} | |} | ||
[[Image:S08_M1D6_WF-PrBYPi.jpg|thumb|left|300px| Wed/Fri gels, 1 of 2]] | [[Image:S08_M1D6_WF-PrBYPi.jpg|thumb|left|300px| Wed/Fri gels, 1 of 2]] | ||
[[Image:S08_M1D6_WF-RG.jpg|thumb|center|300px| Wed/Fri gels, 2 of 2]] | [[Image:S08_M1D6_WF-RG.jpg|thumb|center|300px| Wed/Fri gels, 2 of 2]] | ||
Latest revision as of 06:41, 5 March 2008
Diagnostic Digests
TR lab
Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps.
| Team colour | Digest 1 | Digest 2 |
| Green | XhoI+EcoRV | BamHI+SalI |
| Purple | ||
| Red | EcoRV+NotI | BamHI+XhoI |
| Pink | SalI+EcoRV | NotI+EcoRV |
| Blue | NotI+EcoRV | XhoI+BamHI |
| Yellow | BamH1+Sal1 | EcoRV+Nsi1 |
WF lab
The gels were pretty bright today, including the ladder. For the blue group, the MW ladder is only faintly/partly visible on the original polaroid. The bands seem to fall b/w 4-5 Kbp for the most part, but it's hard to say for sure.
| Team colour | Digest 1 | Digest 2 |
| Green | XbaI/XhoI | BamHI/HsiI |
| Purple | ||
| Red | BamHI/XhoI | BamHI/EcoRV |
| Pink | BamHI/SalI | BamHI/NsiI |
| Blue | BamHI/XhoI | Nsi1/EcoRV |
| Yellow | XbaI/XhoI | BamHI/Nsil |
