Talk:20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6): Difference between revisions
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Line 15: | Line 15: | ||
|- | |- | ||
|Green | |Green | ||
| | |XhoI+EcoRV | ||
| | |BamHI+SalI | ||
|- | |- | ||
|Purple | |Purple | ||
Line 51: | Line 51: | ||
|- | |- | ||
|Green | |Green | ||
| | | XbaI/XhoI | ||
| | | BamHI/HsiI | ||
|- | |- | ||
|Purple | |Purple | ||
Line 59: | Line 59: | ||
|- | |- | ||
| Red | | Red | ||
| | |BamHI/XhoI | ||
| | |BamHI/EcoRV | ||
|- | |- | ||
|Pink | |Pink | ||
Line 71: | Line 71: | ||
|- | |- | ||
|Yellow | |Yellow | ||
| | |XbaI/XhoI | ||
| | |BamHI/Nsil | ||
|} | |} | ||
[[Image:S08_M1D6_WF-PrBYPi.jpg|thumb|left|300px| Wed/Fri gels, 1 of 2]] | [[Image:S08_M1D6_WF-PrBYPi.jpg|thumb|left|300px| Wed/Fri gels, 1 of 2]] | ||
[[Image:S08_M1D6_WF-RG.jpg|thumb|center|300px| Wed/Fri gels, 2 of 2]] | [[Image:S08_M1D6_WF-RG.jpg|thumb|center|300px| Wed/Fri gels, 2 of 2]] |
Latest revision as of 06:41, 5 March 2008
Diagnostic Digests
TR lab
Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps.
Team colour | Digest 1 | Digest 2 |
Green | XhoI+EcoRV | BamHI+SalI |
Purple | ||
Red | EcoRV+NotI | BamHI+XhoI |
Pink | SalI+EcoRV | NotI+EcoRV |
Blue | NotI+EcoRV | XhoI+BamHI |
Yellow | BamH1+Sal1 | EcoRV+Nsi1 |
WF lab
The gels were pretty bright today, including the ladder. For the blue group, the MW ladder is only faintly/partly visible on the original polaroid. The bands seem to fall b/w 4-5 Kbp for the most part, but it's hard to say for sure.
Team colour | Digest 1 | Digest 2 |
Green | XbaI/XhoI | BamHI/HsiI |
Purple | ||
Red | BamHI/XhoI | BamHI/EcoRV |
Pink | BamHI/SalI | BamHI/NsiI |
Blue | BamHI/XhoI | Nsi1/EcoRV |
Yellow | XbaI/XhoI | BamHI/Nsil |