Talk:20.109(S08):Start-up protein engineering (Day1): Difference between revisions
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Eva Klinman (talk | contribs) (→WF lab) |
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|- | |- | ||
|PINK | |PINK | ||
| | |D20->P | ||
|CTTCTCATTATTCCCCAAGGATGGGGACGGCACCATCACC | |CTTCTCATTATTCCCCAAGGATGGGGACGGCACCATCACC | ||
| | |D20->R | ||
|CTTCTCATTATTCCGCAAGGATGGGGACGGCACCATCACC | |CTTCTCATTATTCCGCAAGGATGGGGACGGCACCATCACC | ||
|- | |- | ||
| | |PURPLE | ||
| | |S101->L | ||
| | |GATGGGAACGGCTACATCCTCGCTGCTCAG | ||
| | |S101->T | ||
| | |GATGGGAACGGCTACATCACCGCTGCTCAG | ||
|- | |- | ||
| | |BLUE | ||
| | |N60->E | ||
| | |GATGCTGATGGCGAAGGAACGATTTAC | ||
| | |N60->K | ||
| | |GATGCTGATGGCAAGGGAACGATTTAC | ||
|- | |- | ||
| | |RED | ||
| | |G98->P | ||
| | |GTTTTTGACAAGGATGGGAACCCCTACATCAGCGCTGCTCAG | ||
| | |G98->W | ||
| | |GTTTTTGACAAGGATGGGAACTGGTACATCAGCGCTGCTCAG | ||
|- | |- | ||
| | |Yellow | ||
| | |D56H | ||
| | |5' GAT ATG ATC AAT GAA GTC CAT GCT GAT GGC AAT GGA ACG 3' | ||
| | |D56I | ||
| | |5' GAT ATG ATC AAT GAA GTC ATT GCT GAT GGC AAT GGA ACG 3' | ||
|- | |- | ||
| | |GREEN | ||
| | |K94->H | ||
| | |gcattccgtgtttttgacCACgatgggaacggctacatcagcgc | ||
| | |K94->A | ||
| | |gcattccgtgtttttgacGCGgatgggaacggctacatcagcgc | ||
|- | |- | ||
|} | |} | ||
===All class mutations=== | |||
<code> | |||
1 99 | |||
TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY | |||
P D H E P H D D | |||
R R I K D A K P | |||
K P | |||
H W | |||
100 148 | |||
ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK | |||
L R | |||
T W | |||
</code> |
Latest revision as of 13:07, 5 April 2008
TR lab
Team colour | Mutant 1 | Primer 1 (forward) | Mutant 2 | Primer 2 (forward) |
Red | D133R | GGGAAGCAGATATCGATGGTCGTGGCCAAGTAAACTATGAAG | D133W | GGGAAGCAGATATCGATGGTTGGGGCCAAGTAAACTATGAAGAG |
Purple | Y64P | GCTGATGGCAATGGAACGATTCCCTTTCCTGAATTTCTTACTATG | Y64K | GCTGATGGCAATGGAACGATTAAGTTTCCTGAATTTCTTACTATG |
Blue | G98D | GACAAGGATGGGAACGACTACATCAGCGCTGCTCAGTTACGTC | G98P | GACAAGGATGGGAACCCCTACATCAGCGCTGCTCAGTTACGTC |
Pink | G96D | CGTGTTTTTGACAAGGATGACAACGGCTACATCAGCGCTGC | G96K | GTTTTTGACAAGGATAAGAACGGCTACATCAGCGCTGCTCAG |
Yellow | T28D | CGACAAGGATGGGGACGGCACCATCGACACAAAGGAAC | T28R | GATGGGGACGGCACCATCAGGACAAAGGAACTTGGCACCG |
Green | Y64D | GGCAATGGAACGATTGACTTTCCTGAATTTCTGACTATGATGGC | Y64H | GGCAATGGAACGATTCACTTTCCTGAATTTCTCACTATGATGGC |
WF lab
Team colour | Mutant 1 | Primer 1 (forward) | Mutant 2 | Primer 2 (forward |
PINK | D20->P | CTTCTCATTATTCCCCAAGGATGGGGACGGCACCATCACC | D20->R | CTTCTCATTATTCCGCAAGGATGGGGACGGCACCATCACC |
PURPLE | S101->L | GATGGGAACGGCTACATCCTCGCTGCTCAG | S101->T | GATGGGAACGGCTACATCACCGCTGCTCAG |
BLUE | N60->E | GATGCTGATGGCGAAGGAACGATTTAC | N60->K | GATGCTGATGGCAAGGGAACGATTTAC |
RED | G98->P | GTTTTTGACAAGGATGGGAACCCCTACATCAGCGCTGCTCAG | G98->W | GTTTTTGACAAGGATGGGAACTGGTACATCAGCGCTGCTCAG |
Yellow | D56H | 5' GAT ATG ATC AAT GAA GTC CAT GCT GAT GGC AAT GGA ACG 3' | D56I | 5' GAT ATG ATC AAT GAA GTC ATT GCT GAT GGC AAT GGA ACG 3' |
GREEN | K94->H | gcattccgtgtttttgacCACgatgggaacggctacatcagcgc | K94->A | gcattccgtgtttttgacGCGgatgggaacggctacatcagcgc |
All class mutations
1 99
TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY
P D H E P H D D
R R I K D A K P
K P
H W
100 148
ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
L R
T W