Talk:20.109(S09):Data analysis (Day8): Difference between revisions
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| Line 45: | Line 45: | ||
|Group Colour | |Group Colour | ||
|X#Z Mutant | |X#Z Mutant | ||
|Most believable KD; n for X#Z mutant | |Most believable KD; n '''for X#Z mutant''' | ||
|Most believable KD; n for S101L | |Most believable KD; n '''for S101L''' | ||
|Most believable KD; n for WT | |Most believable KD; n '''for WT''' | ||
|Comments | |Comments | ||
|- | |- | ||
| Line 57: | Line 57: | ||
| Y64D killed calcium response | | Y64D killed calcium response | ||
|- | |- | ||
| | |Yellow | ||
| | |Y64D | ||
| | |N/A | ||
| | |~0.4 μM; ~7 | ||
| | |~0.5 μM; ~3 | ||
| | |No Calcium binding in Y64D | ||
|- | |- | ||
| | |Grey | ||
| | |D22W | ||
| | |N/A | ||
| | | 0.46 μM ; ~ 8 | ||
| | | 0.3 μM; ~ 2 | ||
| | |killed calcium binding | ||
|- | |- | ||
| | |Red | ||
| | |D131G | ||
| | |~ 0.6 μM ; ~ 10.31 | ||
| | |~ 0.45 μM ; ~ 32.7 | ||
| | |~ 0.3 μM ; ~ 2 | ||
| | |D131G altered cooperativity and binding | ||
|- | |- | ||
| | |Green | ||
| | |D133W | ||
| | |0.52 μM ; 0.87 | ||
| | |0.51 μM ; 12.6 | ||
| | |0.51 μM ; 8.9 | ||
| | |D133W altered cooperativity but not binding | ||
|- | |- | ||
| | |Orange | ||
| | |E31I | ||
| | |N/A | ||
| | | 0.49 μM ; ~ 7 | ||
| | | 0.49 μM; ~ 7.6 | ||
| | |E31I killed calcium binding | ||
|- | |- | ||
| | |Blue | ||
| | |D58H | ||
| | |0.41 μM ; ~9.56 | ||
| | |0.29 μM ; ~1.44 | ||
| | |0.50 μM ; ~7.94 | ||
| | |D58H altered cooperativity and binding (increased coop, increased binding) | ||
|- | |- | ||
| | |Purple | ||
| | |D93P | ||
| | | 0.55 μM ; ~2.7 | ||
| | | 0.61 μM ; ~3.6 | ||
| | | 0.60 μM; ~6.3 | ||
| | | D93P increased cooperativity, but decreased binding (sweet) | ||
|- | |- | ||
|} | |} | ||
| Line 141: | Line 134: | ||
|- | |- | ||
|[[Media:S09_M1D7_WF_Orange-1.txt]] <br>[[Media:S09_M1D7_WF_Orange-2.txt]] | |[[Media:S09_M1D7_WF_Orange-1.txt]] <br>[[Media:S09_M1D7_WF_Orange-2.txt]] | ||
| | | [[Media:S09_M1D7_WF_Orange-redo.txt]] | ||
|- | |- | ||
|[[Media:S09_M1D7_WF_Pink.txt]] | |[[Media:S09_M1D7_WF_Pink.txt]] | ||
| Line 153: | Line 146: | ||
|- | |- | ||
|[[Media:S09_M1D7_WF_Yellow.txt]] | |[[Media:S09_M1D7_WF_Yellow.txt]] | ||
| | |[[Media:S09_M1D7_WF_Yellow-redo.txt]] | ||
|- | |- | ||
|} | |} | ||
| Line 162: | Line 155: | ||
|Group Colour | |Group Colour | ||
|X#Z Mutant | |X#Z Mutant | ||
|Most believable KD; n for X#Z mutant | |Most believable KD; n '''for X#Z mutant''' | ||
|Most believable KD; n for S101L | |Most believable KD; n '''for S101L''' | ||
|Most believable KD; n for WT | |Most believable KD; n '''for WT''' | ||
|Comments | |Comments | ||
|- | |- | ||
| Line 170: | Line 163: | ||
|Y64D | |Y64D | ||
| N/A | | N/A | ||
| ~ 0.5; ~ 8 | | ~ 0.5 μM ; ~ 8 | ||
| ~ 0.3; ~ 1(.2) | | ~ 0.3 μM ; ~ 1(.2) | ||
| Y64D killed calcium response | | Y64D killed calcium response | ||
|- | |- | ||
| | |Orange | ||
| | |E127K | ||
| | |Kd-.6285, n=.1490 | ||
| | |N/A (using teaching data) | ||
| | |Kd=.4558, n-1.2418 | ||
| | |S101L and E127K data was noisy | ||
|- | |- | ||
| | |Green | ||
| | |T79P | ||
| | |Kd~0.84 μM, n~2.5 | ||
| | |Kd~0.52 μM, n~7.5 | ||
| | |Kd~0.50 μM, n~7.6 | ||
| | |T79P seems to have decreased cooperativity | ||
|- | |- | ||
| | |Blue | ||
| | |E31R | ||
| | |Kd~0.49 μM, n~0.96 | ||
| | |Used Teaching Sample: Kd~0.5 μM, n~1.1 | ||
| | |Kd~0.45 μM, n~7.4 | ||
| | |E31R decreased calcium binding and cooperativity | ||
|- | |- | ||
| | |Yellow | ||
| | |L105S | ||
| | |Kd~0.33 μM, n~0.722 | ||
| | |Kd~0.51 μM, n~7.8 | ||
| | |Kd~0.69 μM, n~0.147 | ||
| | |L105S and WT-IPC data was noisy. Appears as through L105S decreased cooperativity | ||
|- | |- | ||
| | |Purple | ||
| | |T79P | ||
| | |Kd~.5909 μM, n~3.9182 | ||
| | |Kd~0.2553 μM, n~1.3804 | ||
| | |Kd~.5315 μM, n~5.3068 | ||
| | |T79P decreased cooperativity and affinity | ||
|- | |- | ||
| | |Grey | ||
| | |L32D | ||
| | |Kd~.043μM, n~0.1181 | ||
| | |Kd~.5201μM, n~6.89 | ||
| | |Kd~.2859μM, n~0.5365 | ||
| | |L32D killed calcium response | ||
|- | |- | ||
| | |Pink | ||
| | |M124S | ||
| | |.5080 uM, 14.8384 | ||
| | |.5066 uM, 11.4047 | ||
| | |.6292 uM, 2.2983 | ||
| | |M124S increased binding, increased cooperativity (but our WT Kd is suspiciously high) | ||
|- | |- | ||
| | | | ||
Latest revision as of 23:38, 10 March 2009
T/R data
Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.
Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.
| Day 7 data file | Data taken after 24 hr settling period |
| Media:S09_M1D7_TeachingExample.xls | Mutations: WT, S101L, Y64D |
| Media:S09_M1D7_TR_Blue.txt | |
| Media:S09_M1D7_TR_Green.txt | Media:S09_M1D7_TR_Green-redo.txt |
| Media:S09_M1D7_TR_Grey.txt | Media:S09_M1D7_TR_Grey-redo.txt |
| Media:S09_M1D7_TR_Orange.txt | |
| Media:S09_M1D7_TR_Pink.txt | Media:S09_M1D7_TR_Pink-redo.txt |
| Media:S09_M1D7_TR_Purple.txt | Media:S09_M1D7_TR_Purple-redo.txt |
| Media:S09_M1D7_TR_Red.txt | |
| Media:S09_M1D7_TR_Yellow.txt |
| Group Colour | X#Z Mutant | Most believable KD; n for X#Z mutant | Most believable KD; n for S101L | Most believable KD; n for WT | Comments |
| Teaching Faculty | Y64D | N/A | ~ 0.5 μM ; ~ 8 | ~ 0.3 μM; ~ 1(.2) | Y64D killed calcium response |
| Yellow | Y64D | N/A | ~0.4 μM; ~7 | ~0.5 μM; ~3 | No Calcium binding in Y64D |
| Grey | D22W | N/A | 0.46 μM ; ~ 8 | 0.3 μM; ~ 2 | killed calcium binding |
| Red | D131G | ~ 0.6 μM ; ~ 10.31 | ~ 0.45 μM ; ~ 32.7 | ~ 0.3 μM ; ~ 2 | D131G altered cooperativity and binding |
| Green | D133W | 0.52 μM ; 0.87 | 0.51 μM ; 12.6 | 0.51 μM ; 8.9 | D133W altered cooperativity but not binding |
| Orange | E31I | N/A | 0.49 μM ; ~ 7 | 0.49 μM; ~ 7.6 | E31I killed calcium binding |
| Blue | D58H | 0.41 μM ; ~9.56 | 0.29 μM ; ~1.44 | 0.50 μM ; ~7.94 | D58H altered cooperativity and binding (increased coop, increased binding) |
| Purple | D93P | 0.55 μM ; ~2.7 | 0.61 μM ; ~3.6 | 0.60 μM; ~6.3 | D93P increased cooperativity, but decreased binding (sweet) |
W/F data
Below is your calcium titration data, as well as data from pilot experiments done by the teaching faculty for comparison.
Please fill in the second table by Sunday, if not today, so your classmates can compare their data to yours.
| Group Colour | X#Z Mutant | Most believable KD; n for X#Z mutant | Most believable KD; n for S101L | Most believable KD; n for WT | Comments |
| Teaching Faculty | Y64D | N/A | ~ 0.5 μM ; ~ 8 | ~ 0.3 μM ; ~ 1(.2) | Y64D killed calcium response |
| Orange | E127K | Kd-.6285, n=.1490 | N/A (using teaching data) | Kd=.4558, n-1.2418 | S101L and E127K data was noisy |
| Green | T79P | Kd~0.84 μM, n~2.5 | Kd~0.52 μM, n~7.5 | Kd~0.50 μM, n~7.6 | T79P seems to have decreased cooperativity |
| Blue | E31R | Kd~0.49 μM, n~0.96 | Used Teaching Sample: Kd~0.5 μM, n~1.1 | Kd~0.45 μM, n~7.4 | E31R decreased calcium binding and cooperativity |
| Yellow | L105S | Kd~0.33 μM, n~0.722 | Kd~0.51 μM, n~7.8 | Kd~0.69 μM, n~0.147 | L105S and WT-IPC data was noisy. Appears as through L105S decreased cooperativity |
| Purple | T79P | Kd~.5909 μM, n~3.9182 | Kd~0.2553 μM, n~1.3804 | Kd~.5315 μM, n~5.3068 | T79P decreased cooperativity and affinity |
| Grey | L32D | Kd~.043μM, n~0.1181 | Kd~.5201μM, n~6.89 | Kd~.2859μM, n~0.5365 | L32D killed calcium response |
| Pink | M124S | .5080 uM, 14.8384 | .5066 uM, 11.4047 | .6292 uM, 2.2983 | M124S increased binding, increased cooperativity (but our WT Kd is suspiciously high) |
