Talk:20.109(S09):Induce protein and evaluate DNA (Day5): Difference between revisions
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The table below lists the OD<sub>600</sub> values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations. | The table below lists the OD<sub>600</sub> values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations. | ||
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Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V. | |||
[[Image:S09_M1D3_TR_Yellow.BMP | left |thumb|250px | T/R Gel, Yellow group]] | |||
[[Image:S09_M1D3_TR_Orange.BMP | left |thumb|250px | T/R Gel, Orange group]] | |||
[[Image:S09_M1D3_TR_Blue.BMP | left |thumb|250px | T/R Gel, Blue group]] | |||
[[Image:S09_M1D3_TR_Grey.BMP | left |thumb|250px | T/R Gel, Grey group]] | |||
[[Image:S09_M1D3_TR_Red.BMP | left |thumb|250px | T/R Gel, Red group]] | |||
[[Image:S09_M1D3_TR_Pink.BMP | left |thumb|250px | T/R Gel, Pink group]] | |||
[[Image:S09_M1D3_TR_Green.BMP | left |thumb|250px | T/R Gel, Green group]] | |||
[[Image:S09_M1D3_TR_Purple.BMP | left |thumb|250px | T/R Gel, Purple group]] | |||
There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which will be posted below later tonight. | |||
==WF O/N cultures and Gels== | ==WF O/N cultures and Gels== | ||
Revision as of 14:05, 24 February 2009
TR O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
| Group | OD WT | OD Mut1 | OD Mut2 | Pellets yellow? |
|---|---|---|---|---|
Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V.
There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which will be posted below later tonight.
WF O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
| Group | OD WT | OD Mut1 | OD Mut2 | Pellets yellow? |
|---|---|---|---|---|
pics here
