Talk:20.109(S09):Induce protein and evaluate DNA (Day5)

Examples of induced (left) and control (right) cell pellets

TR O/N cultures and Gels

The table below lists the OD₆₀₀ values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group	OD WT	OD S101L	OD Chosen mutant	Pellets yellow?
Pink	0.117	0.098	0.126	Yes

Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V.

T/R Gel, Yellow group

T/R Gel, Orange group

T/R Gel, Blue group

T/R Gel, Grey group

T/R Gel, Red group

T/R Gel, Pink group

Teaching example

T/R Gel, Green group

T/R Gel, Purple group

There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which is posted above. Unfortunately, some of the bands are faint. Lane 1 is parent IPC (only one form of the plasmid is visible), lane 2 is the ladder, lane 4 is the parent digested with FspI, and lane 8 is the S101L mutant digested with FspI. The short band is very faint, but it is present. I will have the original photo available for viewing for anyone who wants to clearly see the short band.

WF O/N cultures and Gels

The table below lists the OD₆₀₀ values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.

Group	OD WT	OD Mut1	OD Mut2	Pellets yellow?

pics here