Talk:20.109(S09):Start-up protein engineering (Day1): Difference between revisions

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{| border="1"
{| border="1"
|Team colour
|Team colour
|Mutation X#Y
|Mutation X#Z
|Primer (forward)
|Primer (forward)
|Silent mutation
|Silent mutation
Line 9: Line 9:
|-
|-
|Teaching sample
|Teaching sample
|Y64D
|S101L
|GCT GAT GGC AAT GGA ACG ATC GAC TTT CCT GAA TTT CTT ACT ATG
|GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G
|Ile 63
|Ala 103
|PvuI
|FspI
|-
| Red
|D131G
|G ATA AGG GAA GCA GAT ATC GGT GGT GAT GGC CAA GTT AAC TAT
|Val 136
|HpaI
|-
| Orange
|E31I
|C GCG CGC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG<br> Ordered: GC ACC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG AGG
|Leu 32
|XcmI
|-
| Yellow
|D133R
|AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTA AAC TAT GAA GAG <br> Ordered: AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTT AAC TAT GAA GAG
|Val 136
|HpaI
|-
| Green
|D133W
|GG GAA GCA GAT ATC GAT GGT TGG GGC CAA GTT AAC TAT G
|Val 414
|HpaI
|-
| Blue
|D58H
|GAA GTC GAT GCG CAT GGC AAT GGA ACG ATT TAC
|Ala 57
|FspI
|-
| Pink
|M144D
|GAA GAG TTC GTA CAA GAC ATG ACA GCA AAG TAA GAA
|Phe 419, Glu 418
|EcoRI
|-
| Purple
|D93P
|CGA GAA GCT TTC CGT GTT TTT CCC AAG GAT GGG AAC GGC
|Ala 88
|HindIII
|-
| Grey
|D22W
|GCC TTC TCA TTA TTC GAC AAG TGG GGG GAC GGC AAC ATC ACC<br> Ordered: GCC TTC TCA TTA TTC GAC AAG TGG GGA GAC GGC AAC ATC ACC
|Gly 301
|BsmBI
|-
|-
|}
|}
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===WF lab===
===WF lab===
{| border="1"
|Team colour
|Mutation X#Z
|Primer (forward)
|Silent mutation
|New restriction site
|-
|Teaching sample
|S101L
|GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G
|Ala 103
|FspI
|-
| Red
|D93H<br>actual: D95H
|GCA TTC CGT GTT TTT GAC AAG CAT GGG AAC GGA TAC ATC AGT GC
|Gly 379  Ser 388
|BaeI
|-
| Orange
|E127K
|GTT GAT GAA ATG ATC AGG AAA GCA GAT ATC GAT GGT GAT GGC
|Ile 403
|BclI
|-
| Yellow
|L105S
|C AGC GCT GCT CAG TCA CGT CAC GAC ATG ACA AAC <br>Ordered: C AGC GCT GCT CAG TCA CGA CAC GTC ATG ACA AAC
|Arg384
|AflIII
|-
| Green
|T79P
|GA AAA ATG AAG GAC CCA GAC AGC GAA GAG G <br>Ordered: see Green/Purple
|Thr 79
|PpuMI
|-
| Blue
|E31R
|ACC ATC ACC ACA AAG AGG CTG GGC ACC GTT ATG AGG
|Leu 310
|BseYI
|-
| Pink
| M124S
| GAA GTT GAT GAA TCG ATA AGG GAA GCA GAT ATC GAT GG
| none
| ClaI
|-
| Purple
| T79P
|GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC CGA GAA GC<br>Ordered: see Green/Purple
| PpuMI
|
|-
| Grey
|L32D
|GGC ACC ATC ACC ACA AAG GAA GAT GGT ACC GTT ATG AGG
|
|KpnI
|-
| Green/Purple
|T79P
|GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC C
|Thr 79
|PpuMI
|-
|}

Latest revision as of 08:35, 9 February 2009

TR lab

Team colour Mutation X#Z Primer (forward) Silent mutation New restriction site
Teaching sample S101L GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G Ala 103 FspI
Red D131G G ATA AGG GAA GCA GAT ATC GGT GGT GAT GGC CAA GTT AAC TAT Val 136 HpaI
Orange E31I C GCG CGC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG
Ordered: GC ACC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG AGG 		Leu 32 XcmI
Yellow D133R AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTA AAC TAT GAA GAG
Ordered: AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTT AAC TAT GAA GAG 		Val 136 HpaI
Green D133W GG GAA GCA GAT ATC GAT GGT TGG GGC CAA GTT AAC TAT G Val 414 HpaI
Blue D58H GAA GTC GAT GCG CAT GGC AAT GGA ACG ATT TAC Ala 57 FspI
Pink M144D GAA GAG TTC GTA CAA GAC ATG ACA GCA AAG TAA GAA Phe 419, Glu 418 EcoRI
Purple D93P CGA GAA GCT TTC CGT GTT TTT CCC AAG GAT GGG AAC GGC Ala 88 HindIII
Grey D22W GCC TTC TCA TTA TTC GAC AAG TGG GGG GAC GGC AAC ATC ACC
Ordered: GCC TTC TCA TTA TTC GAC AAG TGG GGA GAC GGC AAC ATC ACC 		Gly 301 BsmBI


WF lab

Team colour Mutation X#Z Primer (forward) Silent mutation New restriction site
Teaching sample S101L GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G Ala 103 FspI
Red D93H
actual: D95H 		GCA TTC CGT GTT TTT GAC AAG CAT GGG AAC GGA TAC ATC AGT GC Gly 379 Ser 388 BaeI
Orange E127K GTT GAT GAA ATG ATC AGG AAA GCA GAT ATC GAT GGT GAT GGC Ile 403 BclI
Yellow L105S C AGC GCT GCT CAG TCA CGT CAC GAC ATG ACA AAC
Ordered: C AGC GCT GCT CAG TCA CGA CAC GTC ATG ACA AAC 		Arg384 AflIII
Green T79P GA AAA ATG AAG GAC CCA GAC AGC GAA GAG G
Ordered: see Green/Purple 		Thr 79 PpuMI
Blue E31R ACC ATC ACC ACA AAG AGG CTG GGC ACC GTT ATG AGG Leu 310 BseYI
Pink M124S GAA GTT GAT GAA TCG ATA AGG GAA GCA GAT ATC GAT GG none ClaI
Purple T79P GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC CGA GAA GC
Ordered: see Green/Purple 		PpuMI
Grey L32D GGC ACC ATC ACC ACA AAG GAA GAT GGT ACC GTT ATG AGG KpnI
Green/Purple T79P GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC C Thr 79 PpuMI
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