Talk:20.109(S09):Wrap-up analysis (Day7): Difference between revisions
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Data Summaries: | Data Summaries: | ||
Michael Meyer: [[Media: Module_3_Data_Summary.pdf]] | Michael Meyer: [[Media: Module_3_Data_Summary.pdf]] | ||
Charlotte Yang: [[Media: Final_Report.docx]] | |||
Addendum: The absorbance readings taken before RT-PCR from all of our samples were .001 for 260 nm and 280 nm. Based on the readings alone, we have significant protein contamination. However, we need to keep in mind that measurements this low are not very significant and could easily be off by small amounts, which would throw off the 260 to 280 ratio by a lot. Thus we cannot say for sure whether or not we had protein contamination (from collagen, or even from the alginate). | Addendum: The absorbance readings taken before RT-PCR from all of our samples were .001 for 260 nm and 280 nm. Based on the readings alone, we have significant protein contamination. However, we need to keep in mind that measurements this low are not very significant and could easily be off by small amounts, which would throw off the 260 to 280 ratio by a lot. Thus we cannot say for sure whether or not we had protein contamination (from collagen, or even from the alginate). | ||
Revision as of 11:56, 7 May 2009
T/R data
Data Summaries: Michael Meyer: Media: Module_3_Data_Summary.pdf Charlotte Yang: Media: Final_Report.docx
Addendum: The absorbance readings taken before RT-PCR from all of our samples were .001 for 260 nm and 280 nm. Based on the readings alone, we have significant protein contamination. However, we need to keep in mind that measurements this low are not very significant and could easily be off by small amounts, which would throw off the 260 to 280 ratio by a lot. Thus we cannot say for sure whether or not we had protein contamination (from collagen, or even from the alginate).
