Talk:20.109(S09): Luciferase assays and RNA prep (Day6): Difference between revisions
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==T/R Luciferase Reactions== | ==T/R Luciferase Reactions== | ||
Be sure to indicate if any of your samples were diluted! | Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly). | ||
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from. | |||
{| border="1" | {| border="1" | ||
Line 10: | Line 12: | ||
! colspan="2" | scrambled | ! colspan="2" | scrambled | ||
! colspan="2" | experimental | ! colspan="2" | experimental | ||
! raw or subtracted? | |||
|-- | |-- | ||
| | | | ||
Line 22: | Line 25: | ||
| Rep 1 ratio | | Rep 1 ratio | ||
| Rep 2 ratio | | Rep 2 ratio | ||
| | |||
|-- | |-- | ||
|Pink | |||
|4.5 | |||
|4.0 | |||
|20.7 | |||
|19.1 | |||
|2.73 | |||
|1.92 | |||
|18.9 | |||
|22.9 | |||
|14.7 | |||
|17.0 | |||
| | | | ||
|-- | |||
|Green | |||
|.94 | |||
|.97 | |||
|36.4 | |||
|26.3 | |||
|2.7 | |||
|2.4 | |||
|27.6 | |||
|29.1 | |||
|2.8 | |||
|2.6 | |||
|Raw | |||
|-- | |||
|Orange | |||
|.076 | |||
|22.3 | |||
|21.2 | |||
|29.0 | |||
|2.25 | |||
|1.74 | |||
|22.0 | |||
|22.8 | |||
|4.8 | |||
|4.1 | |||
| | | | ||
|-- | |||
|Yellow | |||
|1.33 | |||
|.17 | |||
|20.8 | |||
|29.4 | |||
|1.42 | |||
|2.24 | |||
|25.74 | |||
|26.08 | |||
|1.17 | |||
|1.53 | |||
| | | | ||
|-- | |||
|Grey | |||
|1.74 | |||
|1.10 | |||
|38.64 | |||
|34.59 | |||
|2.55 | |||
|2.90 | |||
|37.75 | |||
|35.83 | |||
|9.04 | |||
|8.80 | |||
|Raw | |||
|-- | |||
|Red | |||
|2.02 | |||
|3.00 | |||
|21.36 | |||
|16.40 | |||
|1.31 | |||
|1.51 | |||
|20.78 | |||
|23.90 | |||
|1.53 | |||
|1.61 | |||
|Raw | |||
|-- | |||
|Blue | |||
|1.57 | |||
|3.64 | |||
|14.78 | |||
|18.67 | |||
|1.75 | |||
|.51 | |||
|17.40 | |||
|15.16 | |||
|8.25 | |||
|6.87 | |||
| | | | ||
|-- | |||
|Purple | |||
|1.03 | |||
|1.03 | |||
|40.4 | |||
|0.847* (not reliable) | |||
|3.24 | |||
|3.90 | |||
|23.9 | |||
|29.8 | |||
|7.55 | |||
|8.33 | |||
|Raw | |||
|-- | |||
|} | |||
==W/F Luciferase Reactions== | |||
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (''Renilla''/firefly). | |||
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from. | |||
{| border="1" | |||
|'''Team Colour''' | |||
! colspan="2" | none | |||
! colspan="2" | pLuc only | |||
! colspan="2" | validated | |||
! colspan="2" | scrambled | |||
! colspan="2" | experimental | |||
! raw or subtracted? | |||
|-- | |||
| | | | ||
| Rep 1 ratio | |||
| Rep 2 ratio | |||
| Rep 1 ratio | |||
| Rep 2 ratio | |||
| Rep 1 ratio | |||
| Rep 2 ratio | |||
| Rep 1 ratio | |||
| Rep 2 ratio | |||
| Rep 1 ratio | |||
| Rep 2 ratio | |||
| | | | ||
|-- | |-- | ||
| | |Pink | ||
| | |1.83 | ||
| | |1.39 | ||
| | |22.59 | ||
| | |20.86 | ||
| | |2.87 | ||
| | |2.91 | ||
| | |21.06 | ||
| | |21.61 | ||
| | |18.10 | ||
|18.08 | |||
|R A W | |||
|-- | |||
|Orange | |||
|1.11 | |||
|0.89 | |||
|20.56 | |||
|11.30 | |||
|1.70 | |||
|2.30 | |||
|21.71 | |||
|16.40 | |||
|6.42 | |||
|5.13 | |||
| | | | ||
|-- | |-- | ||
| | |Red | ||
| | |2.133 | ||
| | |2.6 | ||
| | |5.924 | ||
| | |14.4 | ||
| | |0.385 | ||
| | |1.636 | ||
| | |20.96 | ||
| | |19.89 | ||
| | |1.96 | ||
|1.72 | |||
| | | | ||
|-- | |-- | ||
| | |Blue | ||
| | |1.019 | ||
| | |0.761658 | ||
| | |16.17009 | ||
| | |19.39064 | ||
| | |2.758433 | ||
| | |8.679711 | ||
| | |15.43884 | ||
| | |10.86473 | ||
| | |14.8144 | ||
| | |12.87329 | ||
|raw! | |||
|-- | |-- | ||
| | |Purple | ||
| | |1.1016 | ||
| | |1.820 | ||
| | |32.05 | ||
| | |30.98 | ||
| | |1.369 | ||
| | |2.073 | ||
| | |35.384 | ||
| | |33.298 | ||
| | |1.189 | ||
| | |1.751 | ||
|raw | |||
|-- | |-- | ||
| | |Green | ||
| | |0.878 | ||
| | |1.028 | ||
| | |31.548 | ||
| | |23.173 | ||
| | |1.562 | ||
| | |3.331 | ||
| | |24.934 | ||
| | |18.317 | ||
| | |4.434 | ||
| | |9.589 | ||
|Raw | |||
|-- | |-- | ||
| | |Yellow | ||
| | |1.071 | ||
| | |0.942 | ||
| | |29.831 | ||
| | |29.021 | ||
| | |3.040 | ||
| | |3.575 | ||
| | |30.356 | ||
| | |31.690 | ||
| | |1.989 | ||
| | |2.203 | ||
|RAW | |||
|-- | |-- | ||
| | |Grey | ||
| | |16.25 | ||
| | |6.42 | ||
| | |22.1 | ||
| | |18.6 | ||
| | |3.19 | ||
| | |4.02 | ||
| | |3.51 | ||
| | |6.14 | ||
| | |3.25 | ||
|8.35 | |||
|subtracted | |||
| | | | ||
|-- | |-- | ||
|} | |} |
Latest revision as of 11:20, 8 April 2009
T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.
Team Colour | none | pLuc only | validated | scrambled | experimental | raw or subtracted? | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | ||
Pink | 4.5 | 4.0 | 20.7 | 19.1 | 2.73 | 1.92 | 18.9 | 22.9 | 14.7 | 17.0 | |
Green | .94 | .97 | 36.4 | 26.3 | 2.7 | 2.4 | 27.6 | 29.1 | 2.8 | 2.6 | Raw |
Orange | .076 | 22.3 | 21.2 | 29.0 | 2.25 | 1.74 | 22.0 | 22.8 | 4.8 | 4.1 | |
Yellow | 1.33 | .17 | 20.8 | 29.4 | 1.42 | 2.24 | 25.74 | 26.08 | 1.17 | 1.53 | |
Grey | 1.74 | 1.10 | 38.64 | 34.59 | 2.55 | 2.90 | 37.75 | 35.83 | 9.04 | 8.80 | Raw |
Red | 2.02 | 3.00 | 21.36 | 16.40 | 1.31 | 1.51 | 20.78 | 23.90 | 1.53 | 1.61 | Raw |
Blue | 1.57 | 3.64 | 14.78 | 18.67 | 1.75 | .51 | 17.40 | 15.16 | 8.25 | 6.87 | |
Purple | 1.03 | 1.03 | 40.4 | 0.847* (not reliable) | 3.24 | 3.90 | 23.9 | 29.8 | 7.55 | 8.33 | Raw |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
Note: feel free to put ratios from either background-subtracted or raw values; they are likely to be quite similar. Hoewever, if you see this note, please mark which kind of data your ratios come from.
Team Colour | none | pLuc only | validated | scrambled | experimental | raw or subtracted? | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |||
Pink | 1.83 | 1.39 | 22.59 | 20.86 | 2.87 | 2.91 | 21.06 | 21.61 | 18.10 | 18.08 | R A W | |
Orange | 1.11 | 0.89 | 20.56 | 11.30 | 1.70 | 2.30 | 21.71 | 16.40 | 6.42 | 5.13 | ||
Red | 2.133 | 2.6 | 5.924 | 14.4 | 0.385 | 1.636 | 20.96 | 19.89 | 1.96 | 1.72 | ||
Blue | 1.019 | 0.761658 | 16.17009 | 19.39064 | 2.758433 | 8.679711 | 15.43884 | 10.86473 | 14.8144 | 12.87329 | raw! | |
Purple | 1.1016 | 1.820 | 32.05 | 30.98 | 1.369 | 2.073 | 35.384 | 33.298 | 1.189 | 1.751 | raw | |
Green | 0.878 | 1.028 | 31.548 | 23.173 | 1.562 | 3.331 | 24.934 | 18.317 | 4.434 | 9.589 | Raw | |
Yellow | 1.071 | 0.942 | 29.831 | 29.021 | 3.040 | 3.575 | 30.356 | 31.690 | 1.989 | 2.203 | RAW | |
Grey | 16.25 | 6.42 | 22.1 | 18.6 | 3.19 | 4.02 | 3.51 | 6.14 | 3.25 | 8.35 | subtracted |