Talk:20.109(S09): Luciferase assays and RNA prep (Day6): Difference between revisions

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Revision as of 13:49, 5 April 2009

T/R Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Team Colour none pLuc only validated scrambled experimental
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 4.5 4.0 20.7 19.1 2.73 1.92 18.9 22.9 14.7 17.0
Green .94 .97 36.4 26.3 2.7 2.4 27.6 29.1 2.8 2.6
Orange .076 22.3 21.2 29.0 2.25 1.74 22.0 22.8 4.8 4.1
Yellow 1.33 .17 20.8 29.4 1.42 2.24 25.74 26.08 1.17 1.53
Grey 1.74 1.10 38.64 34.59 2.55 2.90 37.75 35.83 9.04 8.80

W/F Luciferase Reactions

Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).

Team Colour none pLuc only validated scrambled experimental
Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio Rep 1 ratio Rep 2 ratio
Pink 1.83 1.39 22.59 20.86 2.87 2.91 21.06 21.61 18.10 18.08
Orange 1.11 0.89 20.56 11.30 1.70 2.30 21.71 16.40 6.42 5.13
Red 2.133 2.6 5.924 14.4 0.385 1.636 20.96 19.89 1.96 1.72
Blue 1.019 0.761658 16.17009 19.39064 2.758433 8.679711 15.43884 10.86473 14.8144 12.87329
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