Talk:20.109(S09): Luciferase assays and RNA prep (Day6)
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T/R Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
| Team Colour | none | pLuc only | validated | scrambled | experimental | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |
| Pink | 4.5 | 4.0 | 20.7 | 19.1 | 2.73 | 1.92 | 18.9 | 22.9 | 14.7 | 17.0 |
| Green | .94 | .97 | 36.4 | 26.3 | 2.7 | 2.4 | 27.6 | 29.1 | 2.8 | 2.6 |
| Orange | .076 | 22.3 | 21.2 | 29.0 | 2.25 | 1.74 | 22.0 | 22.8 | 4.8 | 4.1 |
W/F Luciferase Reactions
Be sure to indicate if any of your samples were diluted! Also, double-check that you are taking the correct ratio (Renilla/firefly).
| Team Colour | none | pLuc only | validated | scrambled | experimental | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | Rep 1 ratio | Rep 2 ratio | |
| Pink | 1.83 | 1.39 | 22.59 | 20.86 | 2.87 | 2.91 | 21.06 | 21.61 | 18.10 | 18.08 |
| Orange | 1.11 | 0.89 | 20.56 | 11.30 | 1.70 | 2.30 | 21.71 | 16.40 | 6.42 | 5.13 |
